Chayra Endlessa, - (2024) ANALISIS FILOGENETIK LAMIACEAE BERDASARKAN BARKODE ITS, matK, DAN rbcL SECARA IN SILICO. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Lamiaceae, often utilized in traditional and modern medicine, are vulnerable to adulteration and exploitation. A phylogenetic approach can prevent these issues and conserve Lamiaceae biodiversity. However, reconstructing the phylogeny of Lamiaceae is hindered by morphological similarities, discrepancies between morphological and DNA data, and limited phylogenetic studies on certain genera. This study aimed to obtain phylogenetic information of Lamiaceae based on partial nuclear (ITS) and chloroplast (matK and rbcL) DNA barcodes to prevent adulteration and promote the conservation of Lamiaceae. Genera were obtained from the Barcode of Life Data System. Partial DNA sequences from the NCBI GenBank for 52 species across 11 genera (with Spathodea campanulata as the outgroup) were used. Sequences were aligned and trimmed to reconstruct four maximum parsimony trees using MEGA 11 (three based on one barcode and one combined). Tree analysis included parsimony principle, bootstrapping, consistency index (CI), and retention index (RI). The longest average sequence was found in matK (785.6 bp), highest homology in rbcL (83.5%), and most parsimony-informative sites in ITS (40.3%). Trees had low CI (~0.5) and high RI (>0.5). While individual barcodes showed similar genus groupings, they separated some species from their genera. Combining the barcodes corrected these positions, grouping Lamiaceae into six monophyletic clades (bootstrap >70%): Callicarpa; Scutellaria; Clerodendrum, Lamium, and Stachys; Salvia; Thymus, Origanum, and Mentha; Orthosiphon and Ocimum. The combined tree aids in preventing adulteration and conserving Lamiaceae by providing phylogenetic information on adulterated species, which is useful in identifying adulterants and finding alternative species with similar essential oil properties.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?hl=id&user=LT0NSQcAAAAJ ID SINTA Dosen Pembimbing: Topik Hidayat: 256954 Siti Sriyati: 6004536 |
| Uncontrolled Keywords: | adulteration, DNA barcodes, phylogenetics, Lamiaceae. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan) |
| Depositing User: | Chayra Endlessa |
| Date Deposited: | 11 Jul 2024 02:24 |
| Last Modified: | 11 Jul 2024 02:24 |
| URI: | http://repository.upi.edu/id/eprint/118904 |
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