Dewi, Puri Ratnasari (2017) EKSTRAKSI DAN ANALISIS SENYAWA DARI CIPLUKAN (Physalis angulata). S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Penelitian ini bertujuan untuk mengekstraksi dan menganalisis senyawa dari berbagai bagian tumbuhan ciplukan (Physalisangulata).Tahapan penelitian yang dilakukan meliputi tahapan: (i) pemilihan kondisi ekstrak situmbuhan ciplukan yang meliputipemilihanjenispelarut, waktuekstraksidanjumlahekstraksi; (ii) ekstraksi bagian akar, batang, buah, dan daun ciplukan dengan menggunakan kondisiekstraksi yang telahdipilih; (iii) analisisprofilfitokimiamenggunakan HPLC dengandetektor UV; (iv) sertaidentifikasisenyawadalambagiantumbuhanciplukan yang mengandungsenyawa paling banyakdenganmenggunakan UPLC dengan detector massa ESI-QTOF. Hasil penelitian menunjukkan bahwa kondisi ekstraksi menggunakan ultrasonicator dengan pelarut etanol 100%, selama 30 menit, dandilakukansebanyaktiga kali memberikanhasilekstraksi paling tinggi. Analisisprofilfitokimiamenggunakan HPLC berbagaibagiantumbuhanciplukanmenunjukkanbahwabagiandauntumbuhanciplukanmengandungjumlahsenyawa yang lebihbanyakdenganintensitassenyawa polar yang lebih tinggi dibandingkan bagian tumbuhan lain. Analisis lebih lanjut terhadap bagian daun menggunakan UPLC-ESI-QTOF menunjukkan bahwa terdapat Sembilan puncak senyawa dalam ekstrak daun tumbuhan ciplukan.Berdasarkan data ion indukdanfragmentasinya, lima senyawaberhasildiidentifikasi, yaituduasenyawa flavonoid quercetin-3-O- rutinosidedan 3-O-metilquercetin-O-rutinoside, tiga senyawa with asteroiddarijenisphysalin, yaituphysalin D, physalinG, danphysalinF.;--- This research is to isolate and determine the phytochemicals of various parts of ciplukan plant (Physalisangulata). The reearch consist of four steps i.e.: (i) selection of extraction conditions of ciplukan plants, including the solvent; extraction duration, and a number of extraction; (ii) extraction of the roots, stems, fruits, and leaves parts of ciplukan plant using extraction conditions that have been determined; (iii) analysis of phytochemicals profile using HPLC with UV detector; (iv) identification of compounds in one of the extracts that contained more compounds using UPLC with mass detector ESI-QTOF. The results showed that the extraction conditions using ultrasonicator with 100% ethanol, for 30 minutes, and performed three times giving the highest extraction results. Analysis of HPLC-UV chromatogram showed that leaves contained more compounds than other part. The leaves extract also had higher intensity of polar compounds than others. Based on UPLC-ESI-QTOF, there were nine compounds in leaves extract, of which five of them were determined as quercetin-3-O-rutinoside, 3-O- metilquercetin-O-rutinoside, physalin D, physalin G, and physalin F.
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| Item Type: | Thesis (S1) |
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| Additional Information: | No.panggil : S KIM DEW e-2017; Pembimbing : I.Siti Aisyah. |
| Uncontrolled Keywords: | Ciplukan, Physalis angulata, HPLC, UPLC ESI-QTOF, ultrasonikator, Ciplukan, Physalisangulata, HPLC, ESI-QTOF, ultrasonicator. |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan) |
| Depositing User: | Mrs. Santi Santika |
| Date Deposited: | 25 Jan 2018 05:58 |
| Last Modified: | 25 Jan 2018 05:58 |
| URI: | http://repository.upi.edu/id/eprint/28759 |
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