Datia Siti Nur Lisa, - (2024) KEANEKARAGAMAN GENETIK Chlorella sp. PADA MEDIA LIMBAH TAHU DENGAN METODE KARAKTERISTIK BIOKIMIA DAN RANDOM AMPLIFIED POLYMORPHIC DNA. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Kemampuan adaptasi organisme dipengaruhi oleh adanya seleksi lingkungan yang memengaruhi variabilitas genetik. Chlorella sp., berpotensi sebagai fikoremediator dan untuk dapat memanfaatkan potensinya secara optimal yaitu dengan memahami karakteristik fenotipik dan keanekaragaman genetiknya. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik Chlorella sp. pada media limbah tahu dengan karakteristik biokimia dan PCR-RAPD. Metode penelitian mencakup analisis biokimia (protein, lipid, karbohidrat, klorofil-a, dan karotenoid) dan PCR-RAPD untuk menganalisis variasi genetik. Chlorella sp. dalam media LT OUT 100% + Fe (C) menghasilkan protein tertinggi, media LT OUT 100% + Mg + Fe (D) dengan klorofil-a dan karotenoid tertinggi, dan media LT OUT 100% + Mg + Fe + AF6 A2 (E) menghasilkan karbohidrat tertinggi, dengan lipid tertinggi dihasilkan oleh Chlorella sp. dalam media kontrol/AF6 (A). Variasi genetik Chlorella sp. setiap perlakuan berbeda-beda berdasarkan pola pita, dengan persentase polimorfisme dari OPA-5, OPA-7, dan OPA-11 mencapai 89.3% dan nilai PIC 0.35, menunjukkan polimorfisme tinggi dan primer yang informatif. Perbedaan pola karakteristik biokimia (protein, lipid, karbohidrat, klorofil-a, dan karotenoid) juga tercermin pada variasi genetik hasil amplifikasi PCR-RAPD, melalui dendrogram dan pola pita yang terbentuk menunjukkan bahwa komposisi media LT OUT 100% + Mg + Fe (D) dan LT OUT 100% + Mg + Fe + AF6 A2 (E) menghasilkan variasi genetik yang berbeda daripada Chlorella sp. dalam media kontrol/AF6 (K) dan LT OUT 100% (A), yang menunjukkan semakin kompleks dan bervariasi komposisi media limbah tahu, maka semakin kurang atau jauh kemiripan genetik dengan Chlorella sp. dalam media kontrol/AF6 (K) dan LT OUT 100% (A). The adaptability of organisms is influenced by environmental selection, which in turn affects genetic variability. Chlorella sp. has the potential to be a phycoremediator and can optimize its potential by understanding its phenotypic characteristics and genetic diversity. The objective of this study is to analyze the genetic diversity of Chlorella sp. in tofu waste media using biochemical characteristics and PCR-RAPD. The methods employed biochemical analysis (protein, lipid, carbohydrate, chlorophyll-a, and carotenoid) as well as PCR-RAPD to assess genetic variation. Among the different media tested, Chlorella sp. in LT OUT 100% + Fe (C) media demonstrated the highest protein production, while LT OUT 100% + Mg + Fe (D) media showed the highest levels of chlorophyll-a and carotenoids. In addition, LT OUT 100% + Mg + Fe + AF6 A2 (E) media produced the highest carbohydrate content, while Chlorella sp. in the control/AF6 (A) media exhibited the highest lipid production. The genetic variation of Chlorella sp. varied among the different treatments, as evident from the banding patterns. Notably, the percentage of polymorphism for OPA-5, OPA-7, and OPA-11 reached 89.3%, indicating a high level of polymorphism and informative primers with a PIC value of 0.35. The differences in biochemical characteristics (proteins, lipids, carbohydrates, chlorophyll-a, and carotenoids) were reflected in the genetic variation observed through PCR-RAPD amplification results. The dendrogram and banding patterns formed indicate that the composition of LT OUT 100% + Mg + Fe (D) and LT OUT 100% + Mg + Fe + AF6 A2 (E) media yield genetic variations distinct from those of Chlorella sp. in the control/AF6 (K) and LT OUT 100% (A) media. This suggests that the complexity and variability of tofu waste media composition have an impact on the genetic similarity between Chlorella sp. and the control/AF6 (K) and LT OUT 100% (A) media.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?user=oGoGQscAAAAJ&hl=en ID SINTA Dosen Pembimbing: Diah Kusumawaty: 6005459 Any Aryani: 6038233 |
| Uncontrolled Keywords: | Adaptasi, Chlorella sp., keanekaragaman genetik, media limbah tahu, PCR-RAPD Adaptation, Chlorella sp., genetic diversity, tofu waste water media, PCR-RAPD |
| Subjects: | Q Science > Q Science (General) Q Science > QP Physiology Q Science > QR Microbiology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan) |
| Depositing User: | Datia Siti Nur Lisa |
| Date Deposited: | 09 Sep 2024 01:46 |
| Last Modified: | 09 Sep 2024 01:46 |
| URI: | http://repository.upi.edu/id/eprint/123618 |
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