INDUKSI TUNAS AKSILAR Dendrobium sonia PADA MEDIUM 1/2 MS DENGAN PENAMBAHAN BAP, NAA, DAN AIR KELAPA

Aisha Nadhira Syazwany, - and Adi Rahmat, - and Kusdianti, - (2025) INDUKSI TUNAS AKSILAR Dendrobium sonia PADA MEDIUM 1/2 MS DENGAN PENAMBAHAN BAP, NAA, DAN AIR KELAPA. S1 thesis, Universitas Pendidikan Indonesia.

Abstract

Kultur jaringan sebagai teknik propagasi anggrek memiliki keunggulan dalam kuantitas, kualitas, dan waktu perbanyakan dibandingkan teknik konvensional. Penambahan stimulan organik air kelapa berpotensi mengoptimalkan hasil kultur jaringan. Penelitian ini bertujuan untuk mendapatkan kombinasi paling baik dalam menginduksi tunas aksilar Dendrobium sonia. Pada penelitian ini digunakan tunas aksilar Dendrobium sonia yang dikultur pada medium ½ MS dengan penambahan BAP, NAA, dan air kelapa. Penelitian dengan Rancangan Acak Lengkap (RAL) dengan 24 perlakuan sebanyak 3 faktor, yaitu faktor penambahan BAP (2 dan 3 ppm), NAA (0,5 ppm, 1 ppm, dan 1,5 ppm), serta air kelapa (5%, 10%, dan 15%). Hasil penelitian menunjukkan adanya respons eksplan bertahan hijau, induksi tunas, iniasiasi akar, dan induksi kalus. Penambahan BAP, NAA, dan air kelapa pada medium memberikan respons terhadap induksi tunas aksilar pada konsentrasi 2 ppm dan 3 ppm BAP, 1 ppm dan 1,5 ppm NAA, serta 10% dan 15% air kelapa. Inisiasi akar terdapat pada perlakuan 2 ppm BAP, 1,5 ppm NAA, dan 10% air kelapa. Kalus terinduksi pada kombinasi 2 ppm BAP, 1 ppm NAA, dan 5% air kelapa. Kombinasi tanpa penambahan air kelapa tidak menunjukkan adanya respons tunas, akar, dan kalus. Perlakuan terbaik dalam induksi tunas terdapat pada kombinasi 2 ppm BAP + 1 ppm NAA + 15% air kelapa berdasarkan persentase tunas terinduksi tertinggi (50%) dan waktu kemunculan tunas paling cepat pada 4 minggu setelah tanam. Tissue culture as an orchid propagation technique has advantages in terms of quantity, quality, and propagation time compared to conventional techniques. The addition of coconut water organic stimulants has the potential to optimize tissue culture results. This study aims to determine the best combination for inducing axillary shoots of Dendrobium sonia. In this study, axillary shoots of Dendrobium sonia were cultured on ½ MS medium with the addition of BAP, NAA, and coconut water. The study used a completely randomized design (CRD) with 24 treatments consisting of 3 factors, namely the addition of BAP (2 and 3 ppm), NAA (0.5 ppm, 1 ppm, and 1.5 ppm), and coconut water (5%, 10%, and 15%). The results showed responses in explant survival, shoot induction, root initiation, and callus induction. The addition of BAP, NAA, and coconut water to the medium induced axillary shoot formation at concentrations of 2 ppm and 3 ppm BAP, 1 ppm and 1.5 ppm NAA, and 10% and 15% coconut water. Root initiation occurred in the treatment with 2 ppm BAP, 1.5 ppm NAA, and 10% coconut water. Callus was induced in the combination of 2 ppm BAP, 1 ppm NAA, and 5% coconut water. The combination without the addition of coconut water did not show any response in terms of shoots, roots, and callus. The best treatment for shoot induction was the combination of 2 ppm BAP + 1 ppm NAA + 15% coconut water based on the highest percentage of induced shoots (50%) and the fastest shoot emergence time at 4 weeks after planting.

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Item Type:	Thesis (S1)
Additional Information:	https://scholar.google.com/citations?user=nys2B0EAAAAJ&hl=en&oi=ao ID SINTA Dosen Pembimbing: Adi Rahmat: 5986402 Kusdianti: 6041647
Uncontrolled Keywords:	Kultur jaringan tunas aksilar Anggrek Dendrobium sonia dengan penambahan BAP, NAA, dan Air Kelapa Axillary shoot tissue culture of Dendrobium sonia orchid with the addition of BAP, NAA, and coconut water
Subjects:	Q Science > Q Science (General) Q Science > QA Mathematics
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
Depositing User:	Aisha Nadhira Syazwany
Date Deposited:	10 Sep 2025 03:31
Last Modified:	10 Sep 2025 03:31
URI:	http://repository.upi.edu/id/eprint/138392

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