Salsabila Fitri Pratami, - (2024) OPTIMASI DAN KARAKTERISASI FORMULASI NANOSTRUCTURED LIPID CARRIER DARI L-DOPA-SETIL PALMITAT-ASAM LINOLEAT (NLC-DCL) SEBAGAI KANDIDAT OBAT PARKINSON. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
L-DOPA yang merupakan obat penyakit Parkinson memiliki keterbatasan bioavaibilitas dan stabilitas, sehingga perlu dilakukan enkapsulasi L-DOPA menggunakan sistem Nanostructured Lipid Carrier (NLC). Penelitian ini bertujuan memperoleh formulasi optimum, karakteristik, dan nilai entrapment efficiency dan profil drug release dari produk nanoenkapsulasi L-DOPA menggunakan NLC berbasis setil palmitat dan asam linoleat (NLC-DCL). Sintesis sistem NLC untuk L-DOPA dilakukan dengan menggunakan metode homogenisasi panas dan ultrasonikasi, serta optimasi berdasarkan 2 variabel, yaitu perbandingan komposisi lipid dan massa surfaktan. Karakterisasi NLC-DCL meliputi penentuan ukuran partikel, indeks polidipersitas, dan zeta potensial menggunakan PSA, morfologi partikel menggunakan TEM, gugus fungsi menggunakan FTIR. Penentuan entrapment efficiency dan profil drug release dilakukan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan, formulasi terbaik NLC-DCL terjadi pada komposisi setil palmitat dan asam linoleat 4:6 dengan massa surfaktan sebesar 1,25 gram. Produk NLC-DCL yang diperoleh memiliki ukuran partikel rata-rata sebesar 87,9 nm dengan indeks polidipersitas sebesar 0,185, dan zeta potensial yang terukur sebesar -28,3 mV. Hasil karakterisasi FTIR menunjukkan terjadinya pergeseran pada serapan gugus O-H, N-H, C=O, dan C-O yang mengindikasikan adanya interaksi antara L-DOPA dengan setil palmitat dan asam linoleat. Hasil analisis menggunakan TEM menunjukkan bentuk NLC-DCL spherical dengan kisaran ukuran sebesar 87,5 nm. Persentase entrapment efficiency dari NLC-DCL diperoleh sebesar 75,49%. Profil drug release NLC-DCL menunjukkan pelepasan L-DOPA pada 7 jam pertama mengikuti model Korsmeyer-Peppars. Setelah 24 jam, L-DOPA dilepaskan sebanyak 26,68% pada pH 1,2 dan 36,49% pada pH 7,4 L-DOPA, a drug used for Parkinson's disease, has limitations in bioavailability and stability, necessitating its encapsulation using the Nanostructured Lipid Carrier (NLC) system. This study aims to obtain the optimum formulation, characteristics, entrapment efficiency, and drug release profile of L-DOPA nanoencapsulation products using NLC based on cetyl palmitate and linoleic acid (NLC-DCL). The NLC system synthesis for L-DOPA was carried out using the hot homogenization and ultrasonication method, with optimization based on two variables: the lipid composition ratio and the surfactant mass. NLC-DCL characterization includes determining particle size, polydispersity index, and zeta potential using PSA, particle morphology using TEM, and functional groups using FTIR. Entrapment efficiency and drug release profiles were determined using a UV-Vis spectrophotometer. The results showed that the best NLC-DCL formulation occurred at a cetyl palmitate and linoleic acid composition ratio of 4:6 with a surfactant mass of 1.25 grams. The obtained NLC-DCL product had an average particle size of 87.9 nm with a polydispersity index of 0.185, and a measured zeta potential of -28.3 mV. FTIR characterization results showed shifts in the absorption of O-H, N-H, C=O, and C-O groups, indicating interactions between L-DOPA and cetyl palmitate and linoleic acid. TEM analysis results showed that NLC-DCL had a spherical shape with a size range of 87.5 nm. The entrapment efficiency percentage of NLC-DCL was 75.49%. The drug release profile of NLC-DCL showed that L-DOPA release in the first 7 hours followed the Korsmeyer-Peppas model. After 24 hours, 26.68% of L-DOPA was released at pH 1.2 and 36.49% at pH 7.4.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?view_op=new_profile&hl=en&authuser=1 ID SINTA Dosen Pembimbing: Ratnaningsih Eko Sardjono: 5979503 R. Asep Kadarohman: 5984423 |
| Uncontrolled Keywords: | L-DOPA, Nanostructured Lipid Carrier, Setil Palmitat, Asam Linoleat, Penyakit Parkinson L-DOPA, Nanostructured Lipid Carrier, Cetyl Palmitate, Linoleic Acid, Parkinson’s Disease |
| Subjects: | L Education > L Education (General) Q Science > QD Chemistry |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan) |
| Depositing User: | Salsabila Fitri Pratami |
| Date Deposited: | 11 Sep 2024 23:58 |
| Last Modified: | 11 Sep 2024 23:58 |
| URI: | http://repository.upi.edu/id/eprint/124278 |
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