PERANCANGAN VAKSIN BERBASIS EPITOP VIRUS MONKEYPOX SECARA IN SILICO

Dwi Nur Triharsiwi, - (2023) PERANCANGAN VAKSIN BERBASIS EPITOP VIRUS MONKEYPOX SECARA IN SILICO. S1 thesis, Universitas Pendidikan Indonesia.

Abstract

Wabah penyakit monekypox atau cacar monyet telah dilaporkan terjadi di berbagai
negara, tetapi vaksin spesifik untuk monkeypox belum ditemukan. Penelitian ini
bertujuan untuk menganalisis epitop dengan pendekatan in silico dari virus
monkeypox yang memiliki potensi untuk dikembangkan menjadi vaksin spesifik.
Serangkaian analisis in silico dilakukan, seperti prediksi epitop, analisis karakter
imunologis, penambatan molekuler, simulasi dinamika molekuler, prediksi
interaksi epitop dengan sistem imun, dan prediksi cakupan populasi. Penelitian
menunjukkan bahwa terdapat 4 kandidat epitop untuk MHC I dan 3 kandidat epitop
untuk MHC II yang memiliki kemampuan afinitas tinggi. Hasil akhir penelitian ini
adalah dua rekomendasi epitop yang dapat dikembangkan menjadi vaksin yang
menargetkan respon MHC I dan II. Setiap kandidat epitop memiliki karakter
imunologis, seperti antigen, tidak alergen, tidak toksik, terkonservasi, dan tidak
homolog dengan protein dalam tubuh manusia. Analisis penambatan molekuler
menunjukkan bahwa epitop terbaik yang memiliki nilai binding free energy
terendah, yaitu epitop KQKWRCVVY dengan MHC I -867,7 kkal/mol dan epitop
AVCLLFIQSYSIYEN dengan MHC II -833.5 kkal/mol. Simulasi dinamika
molekuler menunjukkan dua epitop terbaik dapat berinteraksi dengan protein MHC
dengan stabil. Kedua epitop terbaik menunjukkan kemampuan untuk menginduksi
respon imun seluler dan dapat memiliki cakupan sebesar 94,9% dari populasi dunia.
Outbreaks of monekypox have been reported in various countries, but there is no
clinically validated specific vaccine for monkeypox. This study aimed to analyzed
epitopes with an in silico approach from monkeypox viruses which has the potential
to be developed into vaccines. A series of in silico analyzes were performed, such
as prediction of epitopes, analysis of immunological characters, molecular docking,
molecular dynamics simulations, prediction of epitope interactions with the
immune system, and prediction of population coverage. The research showed that
there were 4 MHC I epitope candidates and 3 MHC II epitope candidates that had
high affinity abilities. The result of this research were two recommendations of
epitope which has potential to be developed into vaccine that targeted MHC I and
II responses. Each candidate epitope had immunological characteristics, such as
antigen, non-allergenic, non-toxic, durable, and not homologous to proteins in the
human body. The molecular docking simulation revealed that the best epitope with
the lowest bond free energy value was KQKWRCVVY epitope with MHC I -867.7
kcal/mol and AVCLLFIQSYSIYEN epitope with MHC II -833.5 kcal/mol. The
molecular dynamics simulations demonstrated that the best two epitopes could
stably interacted with MHC proteins. The two best epitopes exhibited the ability to
induce cellular immune responses and 94.9% of the world's population would be
covered.

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Item Type:	Thesis (S1)
Additional Information:	Link Google Scholar: https://scholar.google.com/citations?user=TJ-G98gAAAAJ&hl=en ID SINTA Dosen Pembimbing: Diah Kusumawaty: 6005459 Peristiwati: 6146081
Uncontrolled Keywords:	epitop, in silico, MHC, monkeypox, vaksin epitope, in silico, MHC, monkeypox, vaccine
Subjects:	L Education > L Education (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
Depositing User:	Dwi Nur Triharsiwi
Date Deposited:	07 Sep 2023 03:55
Last Modified:	07 Sep 2023 03:55
URI:	http://repository.upi.edu/id/eprint/103291

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