: KANDUNGAN METABOLIT DAN AKTIVITAS ANTIOKSIDAN PADA TUNAS DAN PLANLET NANAS MADU (Ananas comosus (L.) Merr.)

    Siti Sofiah, - and R. Kusdianti, - and Any Fitriani, - (2025) : KANDUNGAN METABOLIT DAN AKTIVITAS ANTIOKSIDAN PADA TUNAS DAN PLANLET NANAS MADU (Ananas comosus (L.) Merr.). S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Nanas madu (Ananas comosus (L.) Merr.) dikenal kaya akan antioksidan dan memiliki berbagai metabolit serta umum digunakan sebagai obat tradisional. Teknik kultur jaringan digunakan sebagai alternatif budidaya, pemenuhan kebutuhan metabolit, dan langkah mitigasi lingkungan. Informasi ilmiah kandungan metabolit dan aktivitas antioksidan pada tanaman nanas madu selama tahap pertumbuhan dan perkembangan secara in vitro khususnya fase tunas dan planlet belum diketahui. Penelitian bertujuan mendapatkan informasi terkait kandungan metabolit dan aktivitas antioksidan pada tunas dan planlet nanas madu. Tunas dan planlet dengan media dasar Murashige & Skoog diambil dari Laboratorium Kultur Jaringan UPTD Balai Benih Hortikultura Provinsi Jawa Barat, dikeringkan, dan diekstrak dengan metode maserasi menggunakan pelarut etanol p.a 70%. Uji metabolit menggunakan GC-MS dan aktivitas antioksidan dengan metode DPPH. Hasil menunjukkan bahwa pada planlet fase akhir (PAk) mengandung paling banyak metabolit (6) dibandingkan planlet fase awal (PAw) (4) dan tunas (4). Aktivitas antioksidan paling baik terdapat pada planlet fase akhir (PAk) meski termasuk kategori aktivitas antioksidan tidak aktif. Setiap tahap perkembangan memiliki kandungan metabolit yang berbeda-beda dan aktivitas antioksidan semakin baik seiring berkembangnya tanaman nanas madu secara in vitro. Honey pineapple (Ananas comosus (L.) Merr.) is known to be rich in antioxidants and has various metabolites and is commonly used as a traditional medicine. Tissue culture techniques are used as an alternative cultivation, to meet metabolite needs, and as an environmental mitigation measure. Scientific information on metabolite content and antioxidant activity in honey pineapple plants during the growth and development stages in vitro, especially the shoot and plantlet phases, has not been found. This study aims to obtain information related to metabolite content and antioxidant activity in honey pineapple shoots and plantlets. Shoots and plantlets with Murashige & Skoog base media were taken from the Tissue Culture Laboratory of the UPTD Horticultural Seed Center of West Java Province, dried, and extracted by maceration using 70% p.a. ethanol solvent. Metabolite tests used GC-MS and antioxidant activity using the DPPH method. The results showed that the late-phase plantlets (PAk) contained the most metabolites (6) compared to the early-phase plantlets (PAw) (4) and shoots (4). The best antioxidant activity was found in the late-phase plantlets (PAk) even though they were included in the inactive antioxidant activity category. Each developmental stage has different metabolite content and antioxidant activity improves as the honey pineapple plants develop in vitro.

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    Official URL: https://repository.upi.edu
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?user=Siti+Sofiah+2102658=id ID SINTA Dosen Pembimbing: R. Kusdianti: 6041647 Any Fitriani: 6004273
    Uncontrolled Keywords: Ananas, antioksidan, Gas Chromatography-Mass Spectrometry (GC-MS), in vitro, metabolit. Ananas, antioxidant, Gas Chromatography-Mass Spectrometry (GC-MS), in vitro, metabolite.
    Subjects: Q Science > QK Botany
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
    Depositing User: Siti Sofiah
    Date Deposited: 11 Sep 2025 06:03
    Last Modified: 11 Sep 2025 06:03
    URI: http://repository.upi.edu/id/eprint/136738

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