ISOLASI BAKTERI ENDOFIT DAUN BUNGA TELANG (Clitoria ternatea L.) DAN AKTIVITAS ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus

Chersy Tiffany Polandos, - and Any Fitriani, - and Didik Priyandoko, - (2025) ISOLASI BAKTERI ENDOFIT DAUN BUNGA TELANG (Clitoria ternatea L.) DAN AKTIVITAS ANTIBAKTERI TERHADAP Escherichia coli DAN Staphylococcus aureus. S1 thesis, Universitas Pendidikan Indonesia.

Abstract

Bunga Telang (Clitoria ternatea L.) merupakan salah satu tanaman yang memiliki kandungan senyawa antibakteri. Bakteri endofit merupakan bakteri yang hidup dalam suatu tanaman dan memiliki kemampuan menghasilkan senyawa kimia yang serupa dengan tanaman inangnya. Oleh karena itu, penelitian ini bertujuan untuk mengisolasi bakteri endofit dari daun C.ternatea serta mengevaluasi aktivitas antibakterinya terhadap Escherichia coli dan Staphylococcus aureus. Penelitian diawali dengan isolasi bakteri endofit dari daun C.ternatea, diikuti dengan seleksi isolat potensial, identifikasi mikroskopis dan makroskopis, pengukuran kurva tumbuh, ekstraksi, serta pengujian aktivitas antibakteri dengan metode Disk Diffusion Assay (DDA), uji Minimum Inhibitory Concentration (MIC), uji Minimum Bactericidal Concentration (MBC), dan uji Time-Kill. Ekstrak diuji pada konsentrasi 40 mg/mL, 10 mg/mL, dan 2,5 mg/mL, dengan kontrol positif berupa Kloramfenikol 30 mg/mL dan kontrol negatif berupa DMSO 10%. Hasil identifikasi menunjukkan bahwa dua isolat memiliki kemiripan dengan genus Bacillus, dan satu isolat dengan genus Lactobacillus. Zona hambat terbesar terhadap E.coli diperoleh dari isolat D3 pada konsentrasi 40 mg/mL, sedangkan terhadap S.aureus diperoleh dari isolat D12 pada konsentrasi 40 mg/mL. Hasil uji MIC, MBC, serta analisis kurva Time-Kill menunjukkan kesesuaian dengan hasil uji DDA. Dengan demikian, penelitian ini membuktikan bahwa ekstrak supernatan bakteri endofit dari daun C.ternatea memiliki aktivitas antibakteri terhadap E.coli dan S.aureus. Butterfly pea (Clitoria ternatea L.) is one of the plants that contains antibacterial compounds. Endophytic bacteria are bacteria that live in a plant and have the ability to produce chemical compounds similar to their host plant. Therefore, this study aims to isolate endophytic bacteria from C.ternatea leaves and evaluate their antibacterial activity against Escherichia coli and Staphylococcus aureus. The research began with the isolation of endophytic bacteria from C.ternatea leaves, followed by the selection of potential isolates, microscopic and macroscopic identification, growth curve measurement, extraction, and antibacterial activity testing with the disk diffusion assay (DDA) method, Minimum Inhibitory Concentration (MIC) Assay, Minimum Bactericidal Concentration (MBC) Assay, and Time-Kill Assay. Extracts were tested at concentrations of 40 mg/mL, 10 mg/mL, and 2.5 mg/mL, with a positive control of 30 mg/mL Chloramphenicol and a negative control of 10% DMSO. The identification results showed that two isolates were similar to the genus Bacillus, and one isolate to the genus Lactobacillus. The largest inhibition zone against E.coli was obtained from isolate D3 at a concentration of 40 mg/mL, while against S.aureus was obtained from isolate D12 at a concentration of 40 mg/mL. The results of MIC, MBC, and Time-Kill curve analysis showed concordance with the DDA test results. Thus, this study proves that the supernatant extract of endophytic bacteria from C.ternatea leaves has antibacterial activity against E.coli and S.aureus.

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Item Type:	Thesis (S1)
Additional Information:	https://scholar.google.com/citations?hl=en&view_op=list_works&authuser=3&gmla=AH8HC4w9RViVfvE6dU72ddqPVswGC2vE0dHPJVME0IMyy5PJ-tq337oS3UAkUlNJeNkSZ3ogIjr0Hh5RbubsIg&user=qc_YITYAAAAJ ID SINTA Dosen Pembimbing Any Fitriani: 6004273 Didik Priyandoko: 6658318
Uncontrolled Keywords:	Antibakteri, bakteri endofit, Clitoria ternatea, Escherichia coli, Staphylococcus aureus Antibacterial, endophytic bacteria, Clitoria ternatea, Escherichia coli, Staphylococcus aureus
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
Depositing User:	Chersy Tiffany Polandos
Date Deposited:	26 Jun 2025 02:27
Last Modified:	26 Jun 2025 02:27
URI:	http://repository.upi.edu/id/eprint/133882

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