Ratu Dewi Hendriantika, - and Peristiwati, - and RR Hertien Koosbandiah S, - (2025) OPTIMASI PRODUKSI ENZIM SELULASE OLEH JAMUR SELULOLITIK Aspergillus niger PADA SUBSTRAT SERBUK KULIT UBI KAYU (Manihot esculenta). S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Enzim selulase merupakan enzim yang mampu menghidrolisis selulosa menjadi komponen gula seperti glukosa, selobiosa dan selotriosa. Enzim selulase diproduksi karena tingginya kebutuhan sebesar 29,17% pada industri pakan ternak, 14,67% industri tekstil, dan 26,17% industri kertas. Salah satu cara menghasilkan enzim selulase adalah dengan memanfaatkan mikroorganisme selulolitik Aspergillus niger. Penelitian ini, bertujuan untuk mengoptimasi produksi enzim selulase oleh Aspergillus niger melalui tahap fermentasi cair (sub-merged) pada substrat kulit ubi kayu (Manihot esculenta) dengan perlakuan pH 4,5 dan 5,5 serta suhu 29,5 dan 30,5℃. Metode penelitian dilakukan dengan mengisolasi kultur murni Aspergillus niger dari Laboratorium PT. Agritama Sinergi Inovasi. Jamur ditumbuhkan pada media CMC agar untuk uji aktivitas selulolitiknya. Jamur selulolitik yang sudah teruji dapat digunakan dalam fermentasi dalam media yang mengandung serbuk kulit ubi kayu sebagai substrat utama selulosa. Hasil penelitian ini menunjukan bahwa jamur selulolitik Aspergillus niger terbukti berpotensi dalam menghasilkan enzim selulase. Aktivitas enzim tertinggi sebesar 2,003 U/mL dengan biomassa 0,535 mg/ml terdapat pada pH optimal 4,5 dan suhu 30,5℃. Penelitian ini memberikan kontribusi penting dalam melihat optimasi produksi enzim selulase dengan perbedaan pH dan suhu oleh jamur Aspergillus niger pada substrat kulit ubi kayu sebagai dasar untuk pengembangan lebih lanjut dalam pengelolaan limbah organik.
Cellulase enzyme is an enzyme that is able to hydrolyze cellulose into sugar components such as glucose, cellobiose and cellotriose. Cellulase enzyme is produced due to the high demand of 29.17% in the animal feed industry, 14.67% in the textile industry, and 26.17% in the paper industry. One way to produce cellulase enzyme is by utilizing cellulolytic microorganism Aspergillus niger. This study aims to optimize the production of cellulase enzyme by Aspergillus niger through liquid fermentation stage (sub-merged) on cassava peel substrate (Manihot esculenta) with pH 4.5 and 5.5 treatment and temperature of 29.5 and 30.5℃. The research method was carried out by isolating a pure culture of Aspergillus niger from the laboratory of PT Agritama Sinergi Inovasi. The fungus was grown on CMC agar media to test its cellulolytic activity. Cellulolytic fungi that have been tested can be used in fermentation in media containing cassava peel powder as the main substrate of cellulose. The results of this study showed that the cellulolytic fungus Aspergillus niger
proved to have the potential to produce cellulase enzyme. The highest enzyme activity of 2.003 U/mL with 0.535 mg/ml biomass was found at an optimal pH of 4.5 and temperature of 30.5℃. This study makes an important contribution in seeing the optimization of cellulase enzyme production with different pH and temperature by Aspergillus niger fungus on cassava peel substrate as a basis for further development in organic waste management.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?view_op=new_articles&hl=en&imq=Ratu+Dewi+Hendriantika# ID SINTA Dosen Pembimbing: Peristiwati : 6146081 RR Hertien Koosbandiah S: 5995733 |
| Uncontrolled Keywords: | Produksi enzim selulase, jamur selulolitik Aspergillus niger, Substrat Kulit Ubi Kayu. Cellulase enzyme production, Cellulolytic fungus Aspergillus niger, Casava peel substrate |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan) |
| Depositing User: | Ratu Dewi Hendriantika |
| Date Deposited: | 06 May 2025 04:09 |
| Last Modified: | 06 May 2025 04:09 |
| URI: | http://repository.upi.edu/id/eprint/132839 |
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