UJI KEMAMPUAN FORMULA KONSORSIUM BAKTERI RHIZOSFER DALAM BIOREMEDIASI LOGAM KROMIUM SECARA IN VITRO

    Nur Aziema, - (2024) UJI KEMAMPUAN FORMULA KONSORSIUM BAKTERI RHIZOSFER DALAM BIOREMEDIASI LOGAM KROMIUM SECARA IN VITRO. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Industri penyamakan kulit umumnya menggunakan krom sebagai bahan penyamak kulit,
    namun sebagian krom yang digunakan akan menjadi limbah yang dapat mencemari
    lingkungan. Salah satu metode yang dapat dilakukan untuk mengatasi pencemaran
    lingkungan ini adalah metode bioremediasi. Dalam bioremediasi, penggunaan konsorsium
    bakteri lebih efektif dibandingkan dengan isolat tunggal. Tujuan dari penelitian adalah
    untuk mengetahui kemampuan formula konsorsium bakteri dari area rhizosfer dalam
    bioremediasi logam krom. Bakteri diisolasi dari tanah area rhizosfer tumbuhan yang berada
    di sekitar bak penampungan limbah di wilayah Sukaregang, Kabupaten Garut. Bakteri
    diseleksi dengan memilih bakteri yang memiliki resistensi tertinggi lalu diidentifikasi
    secara biokimia. Kemampuan removal logam krom oleh konsorsium ditunjukkan
    berdasarkan kompatibilitas antar bakteri, laju pertumbuhan konsorsium, dan efisiensi
    bioremoval. Hasil uji kompatibilitas menunjukkan bahwa bakteri penyusun konsorsium
    saling bersinergis. Pertumbuhan bakteri dalam konsorsium selama proses bioremoval sama
    tanpa adanya dominansi ataupun interaksi antagonisme dalam konsorsium tersebut.
    Konsorsium dengan kombinasi kelima bakteri dengan genus Pseudomonas, Citrobacter,
    Bacillus, Azotobacter, dan Micrococcus memiliki efisiensi bioremoval tertinggi. Genus
    Pseudomonas dan Micrococcus memiliki peran dominan dalam removal logam krom
    dalam konsorsium. Resistensi bakteri terhadap logam krom tidak sama dengan
    kemampuannya dalam removal logam tersebut. Hal tersebut dilihat dari viabilitas sel yang
    sama namun efisiensi yang berbeda pada konsorsium. Semua bakteri dalam konsorsium
    berinteraksi sinergis dan mampu bertahan dalam kondisi adanya toksisitas logam krom
    namun memiliki kemampuan removal yang berbeda.

    The leather tanning industry typically utilises chromium as a tanning agent. However, a
    proportion of the chrome employed will inevitably become waste material that has the
    potential to pollute the surrounding environment. One potential solution to this
    environmental contamination is the utilisation of bioremediation techniques. The use of a
    bacterial consortium is more effective than that of a single isolate in bioremediation. The
    objective of this study was to ascertain the capacity of a bacterial consortium formula
    derived from the rhizosphere to remediate chromium metal. The bacteria were isolated
    from the soil of the rhizosphere area of plants situated in the vicinity of a waste storage
    basin in the Sukaregang area of Garut Regency. The bacteria were initially screened based
    on their resistance levels and then subjected to biochemical identification. The removal
    ability of chromium metal by the consortium was shown based on the compatibility between
    bacteria, the growth rate of the consortium, and the bioremoval efficiency. The
    compatibility test results demonstrated that the bacterial strains comprising the consortium
    exhibited a synergistic interaction. The growth of bacteria in the consortium during the
    bioremoval process was observed to be uniform, with no evidence of dominance or
    antagonistic interactions within the consortium. The consortium containing a combination
    of five bacterial species belonging to the genera Pseudomonas, Citrobacter, Bacillus,
    Azotobacter, and Micrococcus exhibited the highest bioremoval efficiency. The genera
    Pseudomonas and Micrococcus played a significant role in the removal of chromium metal
    within the consortium. It was important to note that bacterial resistance to chromium metal
    did not necessarily correlate with their ability to remove the metal. This could have been
    observed in the fact that the same cell viability showed different efficiency within the
    consortium. The bacteria in the consortium interacted synergistically and demonstrated
    resistance to chromium metal toxicity, but had different removal abilities.

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    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: ID SINTA Dosen Pembimbing: Wahyu Surakusumah: 6723267 Diah Kusumawaty: 6005459
    Uncontrolled Keywords: Bioremediasi, Konsorsium, Krom, Penyamakan Kulit, Rhizosfer Bioremediation, Consortium, Chromium, Leather Tannery, Rhizosphere
    Subjects: Q Science > Q Science (General)
    Q Science > QR Microbiology
    T Technology > TD Environmental technology. Sanitary engineering
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
    Depositing User: Nur Aziema
    Date Deposited: 26 Sep 2024 04:58
    Last Modified: 26 Sep 2024 04:58
    URI: http://repository.upi.edu/id/eprint/126095

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