RESPONS EKSPLAN DAUN NEPENTHES GYMNAMPHORA PADA MEDIUM 1/2 MS DENGAN PENAMBAHAN 2,4-DIKLOROFENOKSIASETAT DAN AIR KELAPA

Salma Annisa Nur Alifah, - (2024) RESPONS EKSPLAN DAUN NEPENTHES GYMNAMPHORA PADA MEDIUM 1/2 MS DENGAN PENAMBAHAN 2,4-DIKLOROFENOKSIASETAT DAN AIR KELAPA. S1 thesis, Universitas Pendidikan Indonesia.

[img] Text
S_BIO_2008156_Title.pdf

Download (976kB)
[img] Text
S_BIO_2008156_Chapter 1.pdf

Download (525kB)
[img] Text
S_BIO_2008156_Chapter 2.pdf
Restricted to Staf Perpustakaan

Download (847kB)
[img] Text
S_BIO_2008156_Chapter 5.pdf

Download (470kB)
[img] Text
S_BIO_2008156_Chapter 4.pdf
Restricted to Staf Perpustakaan

Download (1MB)
[img] Text
S_BIO_2008156_Chapter 3.pdf

Download (1MB)
[img] Text
S_BIO_2008156_Appendix.pdf
Restricted to Staf Perpustakaan

Download (1MB)
Official URL: https://repository.upi.edu/

Abstract

Nepenthes gymnamphora adalah salah satu species tumbuhan karnivora yang ditemukan di Pulau Jawa dan Sumatera. Tumbuhan ini mengalami kelangkaan akibat tingginya permintaan serta kerusakan habitat, sehingga upaya konservasi perlu segera dilakukan. Kesulitan dalam perbanyakan Nepenthes gymnamphora dapat diatasi dengan teknik kultur jaringan. Namun, penelitian mengenai perbanyakan kultur jaringan khususnya zat pengatur tumbuh yang diperlukan masih terbatas. Zat pengatur tumbuh seperti 2,4-D dan stimulator air kelapa sering digunakan untuk memaksimalkan hasil kultur jaringan. Penelitian ini bertujuan untuk menentukan kombinasi terbaik 2,4-D dan air kelapa dalam merangsang respons eksplan daun Nepenthes gymnamphora menuju pembentukan planlet. Media yang digunakan adalah ½ MS dengan variasi 2,4-D (0 ppm; 0,5 ppm; 1 ppm; 1,5 ppm; 2 ppm) dan air kelapa (0%, 10%, 15%, 20%, 25%). Hasil penelitian menunjukkan adanya perubahan morfologi berupa pelengkungan dan perubahan warna dari hijau menjadi kecoklatan. Konsentrasi 2,4-D memberikan pengaruh signifikan terhadap perubahan morfologi dan warna eksplan, sedangkan air kelapa tidak memberikan pengaruh signifikan. Konsentrasi 2,4-D yang paling mempengaruhi pelengkungan adalah 1,5 ppm, sedangkan tingkat browning paling dipengaruhi oleh konsentrasi 2 ppm. Nepenthes gymnamphora is one of the carnivorous plant species found in Java and Sumatra. This plant is experiencing scarcity due to high demand and habitat destruction, so immediate conservation efforts are needed. Difficulties in propagating Nepenthes gymnamphora can be overcome by tissue culture techniques. However, research on tissue culture propagation, especially the required growth regulators, is still limited. Growth regulators such as 2,4-D and coconut water stimulators are often used to maximize tissue culture results. This study aims to determine the best combination of 2,4-D and coconut water in stimulating the response of Nepenthes gymnamphora leaf explants towards plantlet formation. The media used was ½ MS with variations of 2,4-D (0 ppm; 0.5 ppm; 1 ppm; 1.5 ppm; 2 ppm) and coconut water (0%, 10%, 15%, 20%, 25%). The results showed morphological changes in the form of curvature and color changes from green to brownish. The concentration of 2,4-D gave a significant effect on the morphological and color changes of the explants, while coconut water did not give a significant effect. The concentration of 2,4-D that most affected the curvature was 1,5 ppm, while the browning level was most influenced by the concentration of 2 ppm.

Item Type: Thesis (S1)
Additional Information: https://scholar.google.com/citations?view_op=list_works&hl=en&user=LtZjcisAAAAJ ID SINTA Dosen Pembimbing: Adi Rahmat: 5986402 Tri Suwandi: 6198988
Uncontrolled Keywords: 2,4-Diklorofenoksiasetat (2,4-D), Air Kelapa, Medium ½ MS, Nepenthes gymnamphora. 2,4-Dichlorophenoxyacetic acid, Coconut Water, ½ MS Medium, Nepenthes gymnamphora.
Subjects: S Agriculture > SB Plant culture
Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan)
Depositing User: Salma Annisa Nur Alifah
Date Deposited: 12 Sep 2024 02:34
Last Modified: 12 Sep 2024 02:34
URI: http://repository.upi.edu/id/eprint/124236

Actions (login required)

View Item View Item