Ghaida Nurin Athifah, - (2024) PENGARUH EKSTRAK JAHE MERAH TERHADAP SEL SENESCENCE SERTA EKSPRESI GEN P53 DAN BCL-2 PADA LINI SEL MCF-7. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Kanker payudara adalah jenis kanker yang berkembang di jaringan payudara dan penyebab kematian akibat kanker paling tinggi kelima di seluruh dunia. Perkembangan kanker payudara sangat dipengaruhi oleh aktivasi jalur molekuler seperti gen p53 dan Bcl-2. Pengobatan kanker secara konvensional dapat menyebabkan efek samping bagi penderita. Senyawa 6-gingerol dan 6-shogaol yang terkandung pada jahe merah (Zingiber officinale Roscoe var. rubrum) berpotensi sebagai antikanker. Penelitian ini bertujuan untuk mengetahui pengaruh eksrak jahe merah (EJM) terhadap sel senescence serta ekspresi gen p53 dan Bcl-2 pada lini sel kanker payudara (MCF-7). Penelitian ini dilakukan dengan menginduksi EJM pada lini sel kanker payudara (MCF-7). Pewarnaan β-galactosidase digunakan untuk mengamati dan menghitung lini sel kanker payudara (MCF-7) yang mengalami senescence, dengan konsentrasi EJM 100 µg/mL; 50 µg/mL; 25 µg/mL. Ekspresi gen p53 dan Bcl-2 ditentukan menggunakan qRT-PCR, dengan konsentrasi EJM 175 µg/mL; 350 µg/mL; 700 µg/mL. Data ekspresi gen dianalasis secara statistik menggunakan uji ANOVA dan uji Post Hoc Tukey, sedangkan data hubungan antara ekspresi gen p53 dan Bcl-2 dengan senescence pada sel MCF-7 menggunakan uji Pearson Corellation. EJM dapat menginduksi senescence pada sel MCF-7. EJM meningkatkan ekspresi gen p53 serta menurunkan ekspresi gen Bcl-2 pada lini sel kanker payudara (MCF-7). Terdapat hubungan ekspresi gen p53 dan Bcl-2 dengan penuaan dini pada lini sel kanker payudara (MCF-7). Ekstrak jahe merah terbukti berpotensi sebagai agen antikanker. Breast cancer is a type of cancer that develops in breast tissue and is the fifth highest cause of cancer death worldwide. The development of breast cancer is strongly influenced by the activation of molecular pathways such as the p53 and Bcl-2 genes. Conventional cancer treatment can cause side effects for sufferers. The compounds 6-gingerol and 6-shogaol contained in red ginger (Zingiber officinale Roscoe Var. rubrum) have potential anticancer properties. This study aims to determine the effect of red ginger extract (EJM) on cell senescence and p53 and Bcl-2 gene expression in breast cancer cell lines (MCF-7). This research was carried out by inducing EJM in a breast cancer cell line (MCF-7). β-galactosidase staining was used to observe and quantify the breast cancer cell line (MCF-7) undergoing senescence, with an EJM concentration of 100 µg/mL; 50 µg/mL; 25 µg/mL. The expression of p53 and Bcl-2 genes was determined using qRT-PCR, with an EJM concentration of 175 µg/mL; 350 µg/mL; 700 µg/mL. Gene expression data was analyzed statistically using the ANOVA test and Tukey's Post Hoc test, while data on the relationship between p53 and Bcl-2 gene expression and senescence in MCF-7 cells used the Pearson Corellation test. EJM can induce senescence in MCF-7 cells. EJM increased p53 gene expression as well as decreased Bcl-2 gene expression in breast cancer cell lines (MCF-7). There is a relationship between p53 and Bcl-2 gene expression with premature aging in breast cancer cell lines (MCF-7). Red ginger extract has been proven to have potential as an anticancer agent.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?user=9f6iPXIAAAAJ&hl=en ID SINTA Dosen Pembimbing: Didik Priyandoko: 6658318 Hernawati: 6005188 Wahyu Widowati: 5975784 |
| Uncontrolled Keywords: | Bcl-2, Jahe Merah, Kanker Payudara, p53, Senescence Bcl-2, Breast Cancer, p53, Red Ginger, Senescence |
| Subjects: | Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan) |
| Depositing User: | Ghaida Nurin Athifah |
| Date Deposited: | 10 Sep 2024 09:18 |
| Last Modified: | 10 Sep 2024 09:18 |
| URI: | http://repository.upi.edu/id/eprint/123783 |
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