Nurul Ilma Apriliani, - (2024) BIOREMEDIASI KROMIUM MENGGUNAKAN KOMBINASI DUA RHIZOBAKTERI INDIGENOUS SECARA IN VITRO. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Daerah Sukaregang, Kabupaten Garut yang dikenal dengan industri kerajinan kulitnya, menghadapi pencemaran polusi kromium akibat limbah penyamakan kulit yang tidak dikelola dengan baik, sehingga memengaruhi sumber daya tanah dan air. Daerah dinamis tanah yang dipengaruhi oleh akar tumbuhan atau dikenal sebagai rhizosfer menjadi habitat bagi beragam mikroorganisme dengan kemampuan metabolik unik yang berpotensi besar sebagai agen bioremediasi lingkungan tercemar logam berat. Penelitian ini bertujuan untuk mengevaluasi potensi konsorsium bakteri indigenous dari rhizosfer tanah tercemar kromium sebagai agen bioremediasi. Lima genus bakteri—Pseudomonas, Citrobacter, Bacillus, Azotobacter, dan Micrococcus—diidentifikasi sebagai bakteri resisten kromium secara in vitro. Uji kompatibilitas menunjukkan bahwa lima kombinasi dari genus-genus bakteri ini memperlihatkan sinergi tanpa adanya penghambatan pertumbuhan. Di antara lima kombinasi konsorsium yang diuji (AB, BC, CD, DE, EA), kombinasi DE (Azotobacter dan Micrococcus) menunjukkan pertumbuhan terbaik di bawah cekaman kromium, sementara kombinasi EA (Micrococcus dan Pseudomonas) mencapai persentase penghilangan kromium tertinggi, yaitu 30,78%. Analisis statistik mengungkapkan perbedaan signifikan dalam penghilangan kromium dan populasi bakteri antar kombinasi konsorsium. Namun, analisis regresi linier menunjukkan hubungan lemah antara pertumbuhan bakteri di bawah cekaman kromium dan efisiensi penghilangan kromium, menunjukkan bahwa pertumbuhan bakteri tidak secara signifikan memengaruhi kemampuan bioremediasi. Hasil penelitian ini dapat diaplikasikan untuk pengembangan aplikasi bioteknologi untuk pengolahan limbah berbasis konsorsium bakteri, baik secara in situ maupun ex situ. Sukaregang, known for its leathercraft industry, faces chromium pollution due to poorly managed tannery waste, affecting soil and water resources. The dynamic soil area influenced by plant roots, known as the rhizosphere, becomes a habitat for various microorganisms with unique metabolic abilities, which have significant potential as bioremediation agents for environments contaminated with heavy metals. This study aims to evaluate the potential of indigenous bacterial consortia from the chromium-contaminated rhizosphere as bioremediation agents. Five bacterial genera—Pseudomonas, Citrobacter, Bacillus, Azotobacter, and Micrococcus—were identified as chromium-resistant bacteria in vitro. Compatibility tests showed that the five combinations of these bacterial genera exhibited synergy without any growth inhibition. Among the five consortia combinations tested (AB, BC, CD, DE, EA), combination DE (Azotobacter and Micrococcus) showed the best growth under chromium stress, while combination EA (Micrococcus and Pseudomonas) achieved the highest chromium removal percentage at 30.78%. Statistical analysis revealed significant differences in chromium removal and bacterial populations among the consortia. However, linear regression analysis demonstrated a weak relationship between bacterial growth under chromium stress and chromium removal efficiency, suggesting that growth alone does not significantly impact bioremediation capability. This study suggests that using indigenous rhizobacterial consortia could be a practical approach to remediate chromium pollution in areas like Sukaregang. The results of this research can be applied to the development of biotechnological applications for waste treatment based on bacterial consortia, both in situ and ex situ.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?user=qNX4PiYAAAAJ&hl=en&oi=ao https://scholar.google.co.id/citations?user=a40p67MAAAAJ&hl=en ID SINTA Dosen Pembimbing: Dr. Wahyu Surakusumah, M.T.: 6723267 Dr. Hj. Diah Kusumawaty, M.Si.: 6005459 |
| Uncontrolled Keywords: | penyamakan kulit, bioremediasi, resistensi kromium, rhizobakteri, konsorsium bakteri leather tanning, bioremediation, chromium resistance, rhizobacteria, bacterial consortia |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan) |
| Depositing User: | Nurul Ilma Apriliani |
| Date Deposited: | 12 Sep 2024 09:39 |
| Last Modified: | 12 Sep 2024 09:39 |
| URI: | http://repository.upi.edu/id/eprint/123712 |
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