Amalia Putri Salsabila, - (2024) PERBANDINGAN METABOLIT DAUN KOPI ARABIKA (Coffea arabica) DAN ROBUSTA (Coffea canephora) DENGAN POSISI BERBEDA PADA CABANG. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Kopi arabika (Coffea arabica) dan robusta (Coffea canephora) menjadi jenis tanaman kopi yang paling banyak dibudidayakan, termasuk di wilayah produsen kopi terbesar di Jawa Barat yaitu Kabupaten Bandung. Pemanfaatan dan komersialisasi tanaman kopi hingga saat ini terpaku pada bagian biji kopi saja, sedangkan bagian lain seperti daun kopi masih belum termanfaatkan dengan baik. Penelitian ini bertujuan untuk membandingkan profil metabolit yang terkandung pada daun kopi arabika dan robusta dengan posisi yang berbeda pada cabang. Pengambilan daun dilakukan di Kebun Kopi Kadatuan, Kabupaten Bandung. Daun yang digunakan yaitu daun kopi arabika posisi ke 3-4 (A 3-4) dan ke 5-6 (A 5-6), serta robusta posisi ke 3-4 (R 3-4) dan ke 5-6 (R 5-6) dari pucuk. Daun dikeringkan, dihaluskan, dan diekstrak dengan metode maserasi menggunakan pelarut etanol 70% p.a. Kandungan metabolit daun kopi dianalisis menggunakan instrumen Gas Chromatography–Mass Spectrometry (GC-MS) dan diidentifikasi berdasarkan indeks kesamaannya dengan pustaka National Institute of Standards and Technology (NIST). Hasil penelitian menunjukkan daun kopi A 3-4 mengandung 14 senyawa, sedangkan A 5-6 mengandung 24 senyawa. Daun kopi R 3-4 mengandung 17 senyawa, sedangkan daun R 5-6 mengandung 18 senyawa. Keempat ekstrak daun kopi mengandung 11 senyawa yang sama, dua senyawa diantaranya yang mendominasi yaitu senyawa kafeina dan fitol. Penelitian ini menunjukkan adanya keragaman jenis dan jumlah kandungan metabolit pada daun kopi dengan posisi yang berbeda. Arabica (Coffea arabica) and Robusta (Coffea canephora) coffee are the most widely cultivated types of coffee plants, including in Bandung Regency as the largest coffee producing region in West Java. The utilization and commercialization of coffee plants are still focused on coffee beans, while other parts such as coffee leaves have not been well utilized. This study aims to compare the metabolite profile contained in arabica and robusta coffee leaves at different leaf positions. Leaves was taken from Kebun Kopi Kadatuan, Bandung Regency. Leaves used were arabica at 3-4th position (A 3-4) and 5-6th position (A 5-6), robusta at 3-4th position (R 3-4), and 5-6th position (R 5-6) from the shoots on branches. Coffee leaf were dried, pulverized, and extracted using the maceration method with ethanol solvent p.a. 70%. Metabolite content of coffee leaves was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) instrument and identified based on the similarity index with National Institute of Standards and Technology (NIST) library. The results showed that A 3-4 contained 14 compounds, while A 5-6 contained 24 compounds. R 3-4 coffee leaves contain 17 compounds, while R 5-6 contain 18 compounds. There are 11 metabolite compounds found in all coffee leaf extracts, which two dominating compounds were caffeine and phytol. This study shows the diversity of types and amounts of metabolite content in coffee leaves with different leaf positions.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?hl=en&user=_q1VSVAAAAAJ ID SINTA Dosen Pembimbing Kusdianti: 6041647 Tina Safaria Nilawati: 6798289 |
| Uncontrolled Keywords: | Daun kopi, Gas Chromatography–Mass Spectrometry (GC-MS), metabolit, posisi daun Coffee leaf, Gas Chromatography–Mass Spectrometry (GC-MS), metabolite, leaf position |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan) |
| Depositing User: | Amalia Putri Salsabila |
| Date Deposited: | 10 Sep 2024 18:33 |
| Last Modified: | 10 Sep 2024 18:33 |
| URI: | http://repository.upi.edu/id/eprint/123657 |
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