Ainun Nisa, - (2024) PENGARUH NANOKRISTAL JAHE MERAH TERHADAP SITOTOKSISITAS SERTA EKSPRESI GEN NFKB DAN CASP3 PADA LINI SEL MCF-7. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Kanker payudara adalah penyebab utama kematian pada wanita dengan jumlah kasus tinggi di seluruh dunia, termasuk di Indonesia. Aktivasi jalur molekuler, seperti NFKB dan CASP3, memainkan peran kunci dalam perkembangan kanker ini. Pengobatan konvensional kanker menghadapi tantangan, seperti resistensi obat dan efek samping. Jahe merah (Zingiber officinale var. Rubrum), khususnya senyawa 6-gingerol dan 6-shogaol, menunjukkan potensi antikanker. Pemanfaatan ekstrak rimpang jahe merah yang diformulasikan dalam bentuk nanokristal diharapkan dapat meningkatkan efektivitas terapi dengan memperbaiki kelarutan dan ketersediaan hayati. Penelitian ini dilakukan untuk mengetahui pengaruh nanokristal jahe merah (NKJM) terhadap sitotoksisitas serta ekspresi gen NFKB dan CASP3 pada sel MCF-7. NKJM dibuat di PT Nanotech Herbal Indonesia dengan metode top-down menggunakan High Energy Milling Machine (HEM-Ellipse 3 Dimension). Sitotoksisitas MCF-7 dianalisis dengan metode WST-8, sedangkan ekspresi gen NFKB dan CASP3 pada sel MCF-7 diukur menggunakan RT-qPCR. Data dianalisis menggunakan secara statistik menggunakan analisis varian (ANOVA) satu arah, kemudian dilanjutkan dengan Dunnet T3 Post-Hoc Test dan Tukey HSD Post Hoc Test (P ≤ 0,05). Hasil penelitian menunjukkan bahwa NKJM secara signifikan meningkatkan efek toksik terhadap sel MCF-7 pada konsentrasi 400 µg/mL dengan nilai IC50 sebesar 353,34 μg/mL setelah pemberian NKJM selama 24 jam, menurunkan ekspresi gen NFKB pada konsentrasi 125 µg/mL, dan meningkatkan ekspresi gen CASP3 pada konsentrasi 125 µg/mL pada sel MCF-7 dibandingkan dengan kontrol. Selain itu, ekspresi gen NFKB memiliki korelasi berbanding terbalik yang sangat kuat dengan ekspresi gen CASP3 pada sel MCF-7. Dengan demikian, dapat disimpulkan bahwa NKJM berpotensi untuk digunakan sebagai agen terapi kanker payudara. Breast cancer is the leading cause of death in women with a high number of cases worldwide, including in Indonesia. Activation of molecular pathways, such as NFKB and CASP3, plays a key role in the development of these cancers. Conventional treatments of cancer face challenges, such as drug resistance and side effects. Red ginger (Zingiber officinale var. Rubrum), particularly the compounds 6-gingerol and 6-shogaol, show anticancer potential. Utilization of red ginger rhizome extract formulated in nanocrystal form is expected to increase therapeutic effectiveness by improving solubility and bioavailability. This study was conducted to determine the effect of red ginger nanocrystals (NKJM) on cytotoxicity and gene expression of NFKB and CASP3 in MCF-7 cells. NKJM was made at PT Nanotech Herbal Indonesia by top-down method using High Energy Milling Machine (HEM-Ellipse 3 Dimension). MCF-7 cytotoxicity was analyzed by WST-8 method, while NFKB and CASP3 gene expression in MCF-7 cells was measured using RT-qPCR. Data were statistically analyzed using one-way analysis of variance (ANOVA), followed by Dunnet T3 Post-Hoc Test and Tukey HSD Post Hoc Test (P ≤ 0.05). The results showed that NKJM significantly increased the toxic effect on MCF-7 cells at a concentration of 400 µg/mL with an IC50 value of 353.34 μg/mL after treatment of NKJM for 24 hours, decreased NFKB gene expression at a concentration of 125 µg/mL, and increased CASP3 gene expression at a concentration of 125 µg/mL in MCF-7 cells compared to the control. In addition, NFKB gene expression has a very strong inverse correlation with CASP3 gene expression in MCF-7 cells. Thus, it can be concluded that NKJM has the potential to be used as a potential therapeutic agent for breast cancer.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?hl=en&user=RSrej5oAAAAJ ID SINTA Dosen Pembimbing: Didik Priyandoko: 6658318 Hernawati: 6005188 Wahyu Widowati: 5975784 |
| Uncontrolled Keywords: | CASP3, MCF-7, Nanokristal, NFKB, Sitotoksisitas, Zingiber officinale var. Rubrum CASP3, MCF-7, Nanocrystals, NFKB, Cytotoxicity, Zingiber officinale var. Rubrum |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan) |
| Depositing User: | Ainun Nisa |
| Date Deposited: | 10 Sep 2024 12:15 |
| Last Modified: | 10 Sep 2024 12:15 |
| URI: | http://repository.upi.edu/id/eprint/122830 |
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