Kissi Hannani Fitriani, - (2019) OPTIMASI SINTESIS NANOPARTIKEL PERAK-EKSTRAK BIJI KARABENGUK (Mucuna pruriens L.) (AgMPn) DAN UJI AKTIVITASNYA SEBAGAI ANTI PARKINSON. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
ABSTRAK Karabenguk (Mucuna pruriens L.) telah banyak digunakan sebagai obat dalam penanganan penyakit Parkinson. Pengubahan ukuran partikel ekstrak biji karabenguk menjadi nanopartikel diharapkan dapat meningkatkan aktivitas antiparkinsonnya. Tujuan dari penelitian ini untuk mengetahui hasil optimasi, hasil karakterisasi, dan aktivitas antiparkinson dari nanopartikel perak-ekstrak biji karabenguk (Mucuna pruriens L.) (AgMPn). Tahapan penelitian yang dilakukan yaitu terdiri dari ekstraksi serbuk biji karabenguk, yang dilakukan dengan metode maserasi menggunakan pelarut etanol 96%:aquades (1:1) pada pH 3. Optimasi sintesis dilakukan dengan mereaksikan larutan perak nitrat dan ekstrak biji karabenguk dengan perbandingan massa 1:5 ; 1:3 ; 1:2 ; 3:1 dan 2:1, kemudian dihomogenkan dengan variasi waktu pengadukan 5, 10, 20, 60, dan 80 menit. Karakterisasi AgMPn dilakukan dengan instrumen Fourier Transform Infrared (FTIR) dan Scanning Electron Microscopy-Energy Dispersive XRay (SEM-EDX), dan x-ray diffraction (XRD). Uji aktivitas antiparkinson dari AgMPn dilakukan dengan cara uji katalepsi pada mencit yang diinduksi haloperidol. Uji katalepsi dilakukan pada dosis 5, 10, 15, 20, 25 mg/kg berat badan mencit. Hasil ekstraksi serbuk biji karabenguk diperoleh ekstrak biji karabenguk kering sebanyak 2,68%. Hasil optimasi sintesis AgMPn diperoleh serbuk AgMPn berwarna hitam sebanyak 30,79% pada perbandingan konsentrasi 1:5 dan waktu pengadukan 20 menit. Hasil karakterisasi menggunakan SEM-EDX menunjukkan bahwa AgMPn hasil sintesis berbentuk bulat (spherical) dengan ukuran partikel 50-86 nm dan mengandung unsur C 28,64%, N 16,14%, O 17,63% dan Ag 37,60%. Spektrum FTIR pada daerah sidik jari 464,84 cm-1 menandakan adanya ikatan Ag-O pada AgMPn. Spektrum XRD diketahui bahwa AgMPn tersusun dari nano kristal berstruktur FCC dengan ukuran kristal 27,13 nm. Hasil uji aktivitas antiparkinson pada mencit menunjukkan AgMPn dosis 5, 10, 15, 20, 25 mg/kg berat badan dapat menurunkan gejala katalepsi, dan dari kelima dosis yang diuji, dosis yang paling baik dalam menurunkan gejala katalepsi adalah dosis 5 mg/kg berat badan. Kata kunci: Mucuna pruriens L., antiparkinson, katalepsi, nanopartikel, perak ABSTRACT Velvet bean (Mucuna pruriens L.) has been widely used as a drug for treatment Parkinson disease’s. Transforming the particle size of velvet bean extract to nanoparticles was expected to increase its antiparkinson activity. The purpose of this research was to determine the result of optimization, characterization, and evaluate its antiparkinson activity silver-velvet bean extract nanoparticles (AgMPn). The stages of the research carried out consisted of extraction velvet bean powder, which was carried out by maceration method using ethanol 96%: aquades (1: 1) at pH 3. Optimization of synthesis was carried out by reacting silver nitrate solution and velvet bean extract with a mass ratio of 1:5; 1:3; 1:2; 3:1 and 2:1, then homogenized with a variation of stirring time of 5, 10, 20, 60, and 80 minutes. AgMPn characterization was carried out with Fourier Transform Infrared (FTIR) and Scanning Electron Microscopy-Energy Dispersive X-Ray (SEM-EDX) instruments, and x-ray diffraction (XRD). The evaluation of antiparkinson’s activity of AgMPn was performed through a cataleptic test in haloperidol-induced mice. The catalepsy test was performed at several dosage, i.e 5, 10, 15, 20, and 25 mg/kg body weight. The extraction of velvet bean powder produced a dry extract in 2.68 %. AgMPn obtained from synthesis optimization was a powder with 30.79% of randement at a concentration ratio of 1:5 and stirring time 20 minutes. Based on the characterization using SEM-EDX showed that the synthesized AgMPn was spherical with a particle size of 50-86 nm and contained elements C 28.64%, N 16.14%, O 17.63% and Ag 37.60%. The FTIR spectrum in the fingerprint area of 464.84 cm-1 indicates the interaction of Ag-O bonds in AgMPn. Based on the XRD spectrum known that AgMPn was composed of nanocrystal with FCC structure, the diameter single crystal was 27.13 nm. The results of antiparkinson's test in mice showed AgMPn dose of 5, 10, 15, 20, 25 mg / kg body weight can reduce the symptoms of catalepsy, and based of the five doses tested, the best dosage in reducing the symptoms of catalepsy is a dose of 5 mg / kg by weight body. Keywords: Mucuna pruriens L., antiparkinson, catalepsy, nanoparticles, silver
| Item Type: | Thesis (S1) |
|---|---|
| Subjects: | L Education > L Education (General) |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan) |
| Depositing User: | Kissi Hannani Fitriani |
| Date Deposited: | 06 Jul 2020 06:01 |
| Last Modified: | 06 Jul 2020 06:01 |
| URI: | http://repository.upi.edu/id/eprint/42131 |
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