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<http://repository.upi.edu/id/eprint/76956> <http://purl.org/ontology/bibo/abstract> "Dalam suatu kegiatan budidaya ikan, banyak permasalahan yang dihadapi oleh para pembudidaya ikan salah satunya adalah permasalahan penyakit. Salah satu penyakit yang disebabkan oleh bakteri pada ikan adalah penyakit Motile Aeromonas Septicemia (MAS). MAS merupakan penyakit serius yang menyerang semua jenis ikan air tawar di daerah tropis yang disebabkan oleh bakteri Aeromonas hydrophila. Penyakit ini tidak hanya menginfeksi ikan, namun juga patogen terhadap udang dan amfibi. Tujuan dari penelitian ini yaitu untuk mengembangkan primer multiplex untuk pendeteksian Aeromonas hydrophila patogen. Terdapat empat sampel bakteri Aeromonas hydrophila  dan satu sampel bakteri Aeromonas taiwanensis yang digunakan dalam pengujian ini. Sebelumnya dilakukan perancangan primer secara in silico. Sampel di uji secara molekuler sebagai validasi secara molekuler menggunakan PCR dengan 16S rDNA dan lima gen virulen Aeromonas hydrophila (AerA, alt, ahpA, HlyA, dan ast). Selanjutnya dilakukan uji secara in vitro yaitu pengujian Single dan Multiplex PCR. Hasil amplifikasi seluruh sampel dengan 16S rDNA menghasilkan amplicon 1.500 bp yang menunjukan bahwa sampel yang diuji merupakan mikro organisme. Lima gen virulen primer multiplex dibuat dalam satu set (AerA, alt, ahpA, HlyA, dan ast). Primer dilakukan optimasi secara single PCR dengan rentang Ta 55-65°C dengan Ta yang optimal yaitu 58°C Berdasarkan uji multiplex PCR semua gen yang dirancang berhasil diamplifikasi namun belum ada sampel yang memunculkan ke lima gen yang diuji sehingga penelitian dengan sekuensing pun dibutuhkan sebagai validasi dari sampel yang diuji."^^<http://www.w3.org/2001/XMLSchema#string> .
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<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093969> .
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<http://repository.upi.edu/id/document/2093969> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093788> .
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<http://repository.upi.edu/id/document/2093970> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093970> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093970> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://repository.upi.edu/id/document/2093788> .
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<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093971> .
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<http://repository.upi.edu/id/document/2093971> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093971> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093971> <http://eprints.org/relation/ismediumThumbnailVersionOf> <http://repository.upi.edu/id/document/2093788> .
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<http://repository.upi.edu/76956/14/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093972> .
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<http://repository.upi.edu/id/document/2093972> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093972> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093972> <http://eprints.org/relation/issmallThumbnailVersionOf> <http://repository.upi.edu/id/document/2093788> .
<http://repository.upi.edu/id/document/2093972> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/15/small.jpg> .
<http://repository.upi.edu/id/document/2093972> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/15/small.jpg> .
<http://repository.upi.edu/76956/15/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093973> .
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<http://repository.upi.edu/id/document/2093973> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093973> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093973> <http://eprints.org/relation/islightboxThumbnailVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093973> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/16/lightbox.jpg> .
<http://repository.upi.edu/id/document/2093973> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/16/lightbox.jpg> .
<http://repository.upi.edu/76956/16/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "lightbox.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
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<http://repository.upi.edu/id/document/2093974> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093974> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093974> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093974> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/17/preview.jpg> .
<http://repository.upi.edu/id/document/2093974> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/17/preview.jpg> .
<http://repository.upi.edu/76956/17/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093975> .
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<http://repository.upi.edu/id/document/2093975> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093975> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093975> <http://eprints.org/relation/ismediumThumbnailVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093975> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/18/medium.jpg> .
<http://repository.upi.edu/id/document/2093975> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/18/medium.jpg> .
<http://repository.upi.edu/76956/18/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093976> .
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<http://repository.upi.edu/id/document/2093976> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093976> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093976> <http://eprints.org/relation/issmallThumbnailVersionOf> <http://repository.upi.edu/id/document/2093783> .
<http://repository.upi.edu/id/document/2093976> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/19/small.jpg> .
<http://repository.upi.edu/id/document/2093976> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/19/small.jpg> .
<http://repository.upi.edu/76956/19/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
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<http://repository.upi.edu/id/document/2093977> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093977> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093977> <http://eprints.org/relation/islightboxThumbnailVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093977> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/20/lightbox.jpg> .
<http://repository.upi.edu/id/document/2093977> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/20/lightbox.jpg> .
<http://repository.upi.edu/76956/20/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "lightbox.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093978> .
<http://repository.upi.edu/id/document/2093978> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
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<http://repository.upi.edu/id/document/2093978> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093978> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093978> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093978> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/21/preview.jpg> .
<http://repository.upi.edu/id/document/2093978> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/21/preview.jpg> .
<http://repository.upi.edu/76956/21/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093979> .
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<http://repository.upi.edu/id/document/2093979> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093979> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093979> <http://eprints.org/relation/ismediumThumbnailVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093979> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/22/medium.jpg> .
<http://repository.upi.edu/id/document/2093979> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/22/medium.jpg> .
<http://repository.upi.edu/76956/22/medium.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "medium.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093980> .
<http://repository.upi.edu/id/document/2093980> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
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<http://repository.upi.edu/id/document/2093980> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093980> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093980> <http://eprints.org/relation/issmallThumbnailVersionOf> <http://repository.upi.edu/id/document/2093784> .
<http://repository.upi.edu/id/document/2093980> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/23/small.jpg> .
<http://repository.upi.edu/id/document/2093980> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/23/small.jpg> .
<http://repository.upi.edu/76956/23/small.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "small.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093981> .
<http://repository.upi.edu/id/document/2093981> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
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<http://repository.upi.edu/id/document/2093981> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093981> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093981> <http://eprints.org/relation/islightboxThumbnailVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093981> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/24/lightbox.jpg> .
<http://repository.upi.edu/id/document/2093981> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/24/lightbox.jpg> .
<http://repository.upi.edu/76956/24/lightbox.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "lightbox.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093982> .
<http://repository.upi.edu/id/document/2093982> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> .
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<http://repository.upi.edu/id/document/2093982> <http://eprints.org/relation/isVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093982> <http://eprints.org/relation/isVolatileVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093982> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <http://repository.upi.edu/id/document/2093787> .
<http://repository.upi.edu/id/document/2093982> <http://eprints.org/ontology/hasFile> <http://repository.upi.edu/76956/25/preview.jpg> .
<http://repository.upi.edu/id/document/2093982> <http://purl.org/dc/terms/hasPart> <http://repository.upi.edu/76956/25/preview.jpg> .
<http://repository.upi.edu/76956/25/preview.jpg> <http://www.w3.org/2000/01/rdf-schema#label> "preview.jpg"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://repository.upi.edu/id/eprint/76956> <http://eprints.org/ontology/hasDocument> <http://repository.upi.edu/id/document/2093983> .
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