eprintid: 136852 rev_number: 61 eprint_status: archive userid: 216758 dir: disk0/00/13/68/52 datestamp: 2025-09-02 08:20:56 lastmod: 2025-09-02 08:20:56 status_changed: 2025-09-02 08:20:56 type: thesis metadata_visibility: show creators_name: Ok Muhammad Abthal Al Wafi, - creators_name: Wahyu Surakusumah, - creators_name: Diah Kusumawaty, - creators_nim: NIM2107281 creators_nim: NIDN0003127206 creators_nim: NIDN0011087005 creators_id: okaabthal9@upi.edu creators_id: wahyu_bioupi@upi.edu creators_id: diah.kusumawaty@upi.edu contributors_type: http://www.loc.gov/loc.terms/relators/THS contributors_type: http://www.loc.gov/loc.terms/relators/THS contributors_name: Wahyu Surakusumah, - contributors_name: Diah Kusumawati, - contributors_nidn: NIDN0003127206 contributors_nidn: NIDN0011087005 contributors_id: wahyu_bioupi@upi.edu contributors_id: diah.kusumawaty@upi.edu title: BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA KONSORSIUM BAKTERI BACILLUS LICHENIFORMIS, BACILLUS PARALICHENIFORMIS, DAN BREVIBACILLUS BORSTELENSIS SECARA IN VITRO ispublished: pub subjects: QR subjects: TD divisions: BIO full_text_status: restricted keywords: Bioremedisi, Konsorsium bakteri, Limbah Logam Krom, 16S rRNA, Efisiensi Bioremoval. Bioremediation, Bacterial consortium, Chromium Waste, 16S rRNA, Bioremoval Efficiency. note: https://scholar.google.com/citations?view_op=new_profile&hl=en&authuser=2 ID SINTA Dosen Pembimbing: Wahyu Surakusumah: 6723267 Diah Kusumawaty: 6005459 abstract: Industri penyamakan kulit umumnya menggunakan chromosal B sebagai bahan penyamak kulit yang mengandung logam krom, namun sebagian krom akan terbuang sebagai limbah yang mencemari lingkungan. Salah satu metode penanganannya adalah bioremediasi, di mana penggunaan konsorsium bakteri terbukti lebih efektif dibandingkan isolat tunggal. Penelitian ini bertujuan menentukan kombinasi spesies bakteri paling optimal dalam formula konsorsium sebagai agen bioremediasi logam krom. Bakteri diisolasi dari tanah rizosfer tumbuhan di sekitar bak limbah penyamakan wilayah Sukaregang, Kabupaten Garut. Seleksi dilakukan terhadap isolat yang menunjukkan resistensi tertinggi terhadap krom. Isolat terpilih kemudian diidentifikasi melalui uji morfologi, biokimia, dan analisis molekuler berbasis gen 16S rRNA. Berdasarkan analisis tersebut, diperoleh tiga spesies bakteri: Bacillus licheniformis, Bacillus paralicheniformis, dan Brevibacillus borstelensis. Konsorsium dibentuk berdasarkan uji kompatibilitas dan laju pertumbuhan yang menunjukkan interaksi sinergis antar spesies tanpa dominansi atau antagonisme. Uji efisiensi bioremoval menunjukkan bahwa kombinasi ketiga spesies menghasilkan efisiensi tertinggi sebesar 32,71% dalam inkubasi 14 jam. Spesies B. licheniformis dan B. paralicheniformis berperan dominan dalam proses bioremoval, sementara semua bakteri dalam konsorsium mampu bertahan dalam kondisi toksik logam krom. Temuan ini diharapkan dapat menjadi referensi ilmiah dalam mendukung pengembangan teknologi bioremediasi yang lebih spesifik dan aplikatif untuk menangani pencemaran logam krom, serta menjadi dasar pemanfaatan bakteri resisten sebagai agen bioremediasi pada limbah industri penyamakan kulit. The leather tanning industry commonly uses chromosal B as a tanning agent containing chromium; however, part of this chromium is discharged as waste, polluting the environment. One remediation strategy is bioremediation, in which bacterial consortia have proven more effective than single isolates. This study aimed to determine the optimal combination of bacterial species in a consortium formulation for chromium bioremediation. Bacteria were isolated from rhizosphere soil near tanning wastewater ponds in Sukaregang, Garut Regency. Selection was based on isolates exhibiting the highest chromium resistance. Selected isolates were identified through morphological and biochemical tests, as well as 16S rRNA gene-based molecular analysis. Three species were obtained: Bacillus licheniformis, Bacillus paralicheniformis, and Brevibacillus borstelensis. Consortium formulation was guided by compatibility tests and growth rate assessments, revealing synergistic interactions without dominance or antagonism. Bioremoval tests showed that the three-species combination achieved the highest efficiency of 32.71% after 14 hours of incubation. B. licheniformis and B. paralicheniformis played dominant roles, while all species tolerated chromium toxicity with varying contributions. These findings may serve as a scientific reference for developing targeted and applicable bioremediation technologies to address heavy metal pollution. Furthermore, the study underscores the potential of metal-resistant bacteria as effective bioremediation agents and highlights the value of biochemical and molecular identification in assessing bacterial potential for treating industrial wastewater, particularly chromium-containing leather tanning effluent. date: 2025-08-12 date_type: published institution: Universitas Pendidikan Indonesia department: KODEPRODI46201#Biologi_S1 thesis_type: other thesis_name: other official_url: https://repository.upi.edu/ related_url_url: https://perpustakaan.upi.edu/ related_url_type: org citation: Ok Muhammad Abthal Al Wafi, - and Wahyu Surakusumah, - and Diah Kusumawaty, - (2025) BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA KONSORSIUM BAKTERI BACILLUS LICHENIFORMIS, BACILLUS PARALICHENIFORMIS, DAN BREVIBACILLUS BORSTELENSIS SECARA IN VITRO. S1 thesis, Universitas Pendidikan Indonesia. document_url: http://repository.upi.edu/136852/8/S_BIO_2107281_Title.pdf document_url: http://repository.upi.edu/136852/9/S_BIO_2107281_Chapter1.pdf document_url: http://repository.upi.edu/136852/10/S_BIO_2107281_Chapter2.pdf document_url: http://repository.upi.edu/136852/11/S_BIO_2107281_Chapter3.pdf document_url: http://repository.upi.edu/136852/12/S_BIO_2107281_Chapter4.pdf document_url: http://repository.upi.edu/136852/13/S_BIO_2107281_Chapter5.pdf document_url: http://repository.upi.edu/136852/14/S_BIO_2107281_Appendix.pdf