POTENSI BLEMISH BALM CREAM EKSTRAK KULIT BUAH SALAK (Salacca zalacca (Gaert.) Voss.) TERHADAP EKSPRESI GEN TGF-β1, IL-10, DAN IL-18 PADA MENCIT (Mus musculus L.) YANG DIINDUKSI ULTRAVIOLET SEBAGAI MODEL PENUAAN

    Syifa Indah Suci Ati, - (2023) POTENSI BLEMISH BALM CREAM EKSTRAK KULIT BUAH SALAK (Salacca zalacca (Gaert.) Voss.) TERHADAP EKSPRESI GEN TGF-β1, IL-10, DAN IL-18 PADA MENCIT (Mus musculus L.) YANG DIINDUKSI ULTRAVIOLET SEBAGAI MODEL PENUAAN. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Sinar ultraviolet (UV) merupakan salah satu faktor ekstrinsik utama yang
    menyebabkan penuaan dini pada kulit. Sinar UV mempengaruhi ekspresi gen
    transforming growth factor-beta (TGF-β1), interleukin-10 (IL-10) dan interleukin�18 (IL-18). Penggunaan krim blemish balm (BB) dari ekstrak kulit buah salak
    (Salacca zalacca (Gaert) Voss.) diidentifikasi dapat digunakan sebagai produk
    antipenuaan karena mengandung senyawa antioksidan. Penelitian ini bertujuan
    untuk mengetahui potensi krim BB ekstrak kulit buah salak sebagai antipenuaan
    pada mencit (Mus musculus) model penuaan. Mencit jantan galur DDY diberi
    perlakuan lalu diinduksi UV selama 14 hari. Kelompok uji terdapat enam perlakuan
    yaitu kontrol positif, kontrol negatif, kontrol vehicle, kontrol pembanding, krim
    ekstrak kulit buah salak satu kali olesan (KSI), dan krim ekstrak kulit buah salak
    dua kali (KSII) dengan empat pengulangan. Metode quantitative real–time
    polymerase chain reaction (qRT-PCR) digunakan untuk mengukur ekspresi gen
    TGF-β1, IL-10, dan IL-18 pada kulit mencit. Hasil data dianalisis secara statistik
    dengan uji ANOVA dan dilanjutkan dengan Tukey Post Hoc test p< 0.05. Hasil
    menunjukkan bahwa pemberian krim BB ekstrak kulit buah salak sebanyak dua kali
    olesan secara signifikan dapat mencegah kemerahan, eritema, dan pigmentasi tidak
    merata pada kulit. Secara molekuler pemberian krim BB dua kali olesan mampu
    meningkatkan ekspresi gen TGF-β1 dan IL-10 serta menurunkan ekspresi gen IL�18 dibandingkan kontrol positif. Simpulan penelitian ini adalah krim BB ekstrak
    kulit buah salak memiliki potensi sebagai antipenuaan

    Ultraviolet (UV) rays are one of the main extrinsic factors that cause premature
    aging of the skin. UV light affects the expression of transforming growth factor beta
    (TGF-1), interleukin-10 (IL-10), and interleukin-18 (IL-18) genes. The use of
    blemish balm (BB) cream salak (Salacca zalacca (Gaert) Voss.) peel extract is
    identified as an anti-aging product because it contains antioxidant compounds. This
    study aims to determine the potential of BB cream salak peel extract as an anti�aging agent in old mice (Mus musculus). DDY male mice were treated and then
    UV-induced for 14 days. In the test group, there were six treatments, namely
    positive control, negative control, vehicle control, comparison control, one time
    application of BB cream salak peel extract (KSI), and two time application of BB
    cream salak peel extract (KSII), with four replication. The quantitative real–time
    polymerase chain reaction (qRT-PCR) method was used to measure the expression
    of the TGF-1, IL-10, and IL-18 genes in the skin of mice. The results of the data
    were analyzed statistically with the ANOVA test and continued with the Tukey post
    hoc test p<0.05. The research showed that two application of BB cream salak peel
    can significantly prevented redness, erythema and uneven pigmentation of the skin.
    Molecularly, administration of BB cream salak peel extract was able to increase
    TGF-1 and IL-10 gene expression twice as well as decrease IL-18 gene expression
    compared to positive controls. The conclusion of this research is BB cream salak
    peel extract has potential as an anti-aging agent

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    Official URL: http://repository.upi.edu
    Item Type: Thesis (S1)
    Additional Information: SINTA ID: 6041647 SINTA ID: 6005459
    Uncontrolled Keywords: Antiinflamasi, Antioksidan, Krim BB, Kulit Buah Salak, Penuaan Aging, Anti-inflammatory, Antioxidant, BB Cream, Salak Peel
    Subjects: L Education > L Education (General)
    Q Science > QH Natural history > QH301 Biology
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1
    Depositing User: Syifa Indah Suci Ati
    Date Deposited: 21 Aug 2023 08:13
    Last Modified: 21 Aug 2023 08:13
    URI: http://repository.upi.edu/id/eprint/96607

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