Syifa Indah Suci Ati, - (2023) POTENSI BLEMISH BALM CREAM EKSTRAK KULIT BUAH SALAK (Salacca zalacca (Gaert.) Voss.) TERHADAP EKSPRESI GEN TGF-β1, IL-10, DAN IL-18 PADA MENCIT (Mus musculus L.) YANG DIINDUKSI ULTRAVIOLET SEBAGAI MODEL PENUAAN. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Sinar ultraviolet (UV) merupakan salah satu faktor ekstrinsik utama yang
menyebabkan penuaan dini pada kulit. Sinar UV mempengaruhi ekspresi gen
transforming growth factor-beta (TGF-β1), interleukin-10 (IL-10) dan interleukin�18 (IL-18). Penggunaan krim blemish balm (BB) dari ekstrak kulit buah salak
(Salacca zalacca (Gaert) Voss.) diidentifikasi dapat digunakan sebagai produk
antipenuaan karena mengandung senyawa antioksidan. Penelitian ini bertujuan
untuk mengetahui potensi krim BB ekstrak kulit buah salak sebagai antipenuaan
pada mencit (Mus musculus) model penuaan. Mencit jantan galur DDY diberi
perlakuan lalu diinduksi UV selama 14 hari. Kelompok uji terdapat enam perlakuan
yaitu kontrol positif, kontrol negatif, kontrol vehicle, kontrol pembanding, krim
ekstrak kulit buah salak satu kali olesan (KSI), dan krim ekstrak kulit buah salak
dua kali (KSII) dengan empat pengulangan. Metode quantitative real–time
polymerase chain reaction (qRT-PCR) digunakan untuk mengukur ekspresi gen
TGF-β1, IL-10, dan IL-18 pada kulit mencit. Hasil data dianalisis secara statistik
dengan uji ANOVA dan dilanjutkan dengan Tukey Post Hoc test p< 0.05. Hasil
menunjukkan bahwa pemberian krim BB ekstrak kulit buah salak sebanyak dua kali
olesan secara signifikan dapat mencegah kemerahan, eritema, dan pigmentasi tidak
merata pada kulit. Secara molekuler pemberian krim BB dua kali olesan mampu
meningkatkan ekspresi gen TGF-β1 dan IL-10 serta menurunkan ekspresi gen IL�18 dibandingkan kontrol positif. Simpulan penelitian ini adalah krim BB ekstrak
kulit buah salak memiliki potensi sebagai antipenuaan
Ultraviolet (UV) rays are one of the main extrinsic factors that cause premature
aging of the skin. UV light affects the expression of transforming growth factor beta
(TGF-1), interleukin-10 (IL-10), and interleukin-18 (IL-18) genes. The use of
blemish balm (BB) cream salak (Salacca zalacca (Gaert) Voss.) peel extract is
identified as an anti-aging product because it contains antioxidant compounds. This
study aims to determine the potential of BB cream salak peel extract as an anti�aging agent in old mice (Mus musculus). DDY male mice were treated and then
UV-induced for 14 days. In the test group, there were six treatments, namely
positive control, negative control, vehicle control, comparison control, one time
application of BB cream salak peel extract (KSI), and two time application of BB
cream salak peel extract (KSII), with four replication. The quantitative real–time
polymerase chain reaction (qRT-PCR) method was used to measure the expression
of the TGF-1, IL-10, and IL-18 genes in the skin of mice. The results of the data
were analyzed statistically with the ANOVA test and continued with the Tukey post
hoc test p<0.05. The research showed that two application of BB cream salak peel
can significantly prevented redness, erythema and uneven pigmentation of the skin.
Molecularly, administration of BB cream salak peel extract was able to increase
TGF-1 and IL-10 gene expression twice as well as decrease IL-18 gene expression
compared to positive controls. The conclusion of this research is BB cream salak
peel extract has potential as an anti-aging agent
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | SINTA ID: 6041647 SINTA ID: 6005459 |
| Uncontrolled Keywords: | Antiinflamasi, Antioksidan, Krim BB, Kulit Buah Salak, Penuaan Aging, Anti-inflammatory, Antioxidant, BB Cream, Salak Peel |
| Subjects: | L Education > L Education (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 |
| Depositing User: | Syifa Indah Suci Ati |
| Date Deposited: | 21 Aug 2023 08:13 |
| Last Modified: | 21 Aug 2023 08:13 |
| URI: | http://repository.upi.edu/id/eprint/96607 |
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