NANOENKAPSULASI EKSTRAK BIJI KARABENGUK (Mucuna pruriens) DALAM ASAM PALMITAT SEBAGAI KANDIDAT OBAT ANTIPARKINSON

Ade Indri Jamiati, - (2022) NANOENKAPSULASI EKSTRAK BIJI KARABENGUK (Mucuna pruriens) DALAM ASAM PALMITAT SEBAGAI KANDIDAT OBAT ANTIPARKINSON. S1 thesis, Universitas Pendidikan Indonesia.

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Abstract

ABSTRAK Penyakit parkinson merupakan penyakit neurodegenerative yang disebabkan terjadinya penurunan kadar dopamin di otak. Pengobatan Parkinson dilakukan dengan memberikan L-dopa yang merupakan senyawa prekursor dopamin. Secara alami L-dopa dapat diperoleh dari biji Karabenguk (Mucuna pruriens). Penelitian ini bertujuan untuk memperoleh kondisi optimum proses nanoenkapsulasi ekstrak karabenguk dalam asam palmitat, mengetahui karakteristik, efisiensi enkapsulasi serta kemampuan drug-release produk nanoenkapsulasi tersebut. Proses nanoenkapsulasi dilakukan menggunakan metode modifikasi difusi pelarut dengan variasi komposisi massa pereaksi, amplitudo ultrasonikasi, dan durasi ultrasonikasi. Karakterisasi produk nanoenkapsulasi menggunakan spektroskopi Fourier Transformation Infrared (FTIR), Scanning Electron Microscopy (SEM), dan Transmission Electron Microscopy (TEM). Uji efisiensi enkapsulasi dilakukan dengan mengukur perubahan konsentrasi supernatant ekstrak awal dan konsentrasi supernatan ekstrak setelah dienkapsulasi menggunakan spektrometer UV-Vis. Uji drug release dilakukan menggunakan membran dialisis Cellu Sep T4 pada pH 1,2 dan pH 7,4 selama 5 jam. Berdasarkan penelitian yang dilakukan, kondisi optimum berdasarkan massa produk nanoenkapsulasi terbanyak dihasilkan pada kondisi komposisi massa asam palmitat dengan ekstrak sebesar 25:1, amplitudo ultrasonikasi 85% dan durasi ultrasonikasi selama 25 menit. Hasil karakterisasi menggunakan SEM menunjukkan produk nanoenkapsulasi mempunyai morfologi partikel berbentuk flake (serpihan), sedang hasil analisis TEM menunjukkan ukuran partikel berkisar 13,16-213,82 nm. Hasil karakterisasi FTIR mengindikasikan terjadinya interaksi antara ekstrak karabenguk dengan asam palmitat pada gugus karbonil C=O dengan terjadinya pergeseran pada dari 1701,29 cm-1 menjadi 1699,34 cm-1. Nilai efisiensi enkapsulasi produk nanoenkapsulasi sebesar 52,25% dengan kemampuan drug-release setelah 5 jam pada pH 1,2 adalah 16,7% dan pada pH 7,4 sebesar 61%. Berdasarkan data hasil penelitian, penggunan asam palmitat sebagai enkapsulan memiliki potensi sebagai drug delivery ekstrak karabenguk untuk pengobatan penyakit Parkinson. ABSTRACT Parkinson's disease is a neurodegenerative disease caused by a decrease in dopamine levels in the brain. Parkinson's treatment is done by giving L-dopa which is a dopamine precursor compound. Naturally L-dopa can be obtained from the seeds of Karabenguk (Mucuna pruriens). This study aims to obtain the optimum conditions for the nanoencapsulation of karabenuk extract in palmitic acid, to determine the characteristics, encapsulation efficiency and drug release ability of the nanoencapsulated product. The nanoencapsulation process was carried out using a modified solvent diffusion method with variations in the composition of the reagent mass, ultrasonication amplitude, and ultrasonication duration. Characterization of nanoencapsulated products using Fourier Transformation Infrared (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). Encapsulation efficiency test was carried out by measuring the change in the initial supernatant concentration and supernatant extract concentration after encapsulation using a UV-Vis spectrometer. Drug release test was carried out using Cellu Sep T4 dialysis membrane at pH 1.2 and pH 7.4 for 5 hours. Based on the research conducted, the optimum conditions were based on the maximum mass of nanoencapsulated product produced under conditions of palmitic acid mass composition with extract of 25:1, ultrasonication amplitude of 85% and ultrasonication duration of 25 minutes. The results of the characterization using SEM showed that the nanoencapsulated product had a flake-shaped morphology, while the results of the TEM analysis showed that the particle size ranged from 13.16-213.82 nm. The results of the FTIR characterization showed the interaction between carbeneuk extract and palmitic acid on the C=O carbonyl group with a shift from 1701.29 cm-1 to 1699.34 cm-1. The encapsulation efficiency value of the nanoencapsulated product was 52.25% with drug-release ability after 5 hours at pH 1.2 was 16.7% and at pH 7.4 was 61%. Based on the research data, the use of palmitic acid as an encapsulant has the potential as a drug delivery extract of karabenuk for the treatment of Parkinson's disease.

Item Type:	Thesis (S1)
Additional Information:	Link Google Scholar: https://scholar.google.com/citations?view_op=list_works&hl=en&user=LxZ98nYAAAAJ#d=gs_hdr_drw&t=1663253494709 ID Shinta dosen pembimbing: Ratnaningsih Eko Sardjono: 5979503 Asep Kadarohman : 5984423
Uncontrolled Keywords:	nanoenkapsulasi, karabenguk, asam palmitat, efisiensi enkpasulasi, drug- release
Subjects:	L Education > L Education (General) Q Science > QD Chemistry
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan)
Depositing User:	Ade Indri Jamiati
Date Deposited:	16 Sep 2022 03:30
Last Modified:	16 Sep 2022 03:30
URI:	http://repository.upi.edu/id/eprint/81118

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