STRUKTUR DAN PERKEMBANGAN KALUS TEBU (Saccharum officinarum L.) HASIL SUBKULTUR

    Yudo Sudickyo, - (2008) STRUKTUR DAN PERKEMBANGAN KALUS TEBU (Saccharum officinarum L.) HASIL SUBKULTUR. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Structure and development of sugarcane callus resulted by subculture on agar
    solidified Murashige & Skoog (MS) medium supplemented with 3 mg/1 2,4-
    Diclorophenoxyacetk acid (2,4-D) and 10 %of coconut milk has been observed.
    The callus inducted from young leaf explant of sugarcane var. Ps-851, incubated
    both the dark and underlight To observe the anatomical structure, callus obtained
    from 0-31 days incubation were fixed by 50 % FAA and cut using paraffin
    method Callus was stained with Mayer's Hemalum. Morphological observation
    showed that under light cultured callus has white-brownish color with
    mucilaginous texture. In other hand, the dark cultured callus has white-yellownish
    color with friable texture.S Anatomically, the calli had more meristematic cells in
    outside area than inside area. After subcultured, compact structure were formed on
    the outside and insides area. In darkness, compact structure on the outsides area
    developed into globular embryo like structure. Some calli with compact structure
    incubated on underlight, developed into meristemoid form, then meristematic
    dome and develop into shoot buds like structure at last. Morphologically, this
    structure had form green nodules in surface ofcalli.

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    Official URL: http://repository.upi.edu
    Item Type: Thesis (S1)
    Uncontrolled Keywords: struktur dan perkembangan kalus tebu
    Subjects: L Education > L Education (General)
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Pendidikan Biologi
    Depositing User: Moh. Rizal Syahrial
    Date Deposited: 29 Dec 2022 08:27
    Last Modified: 29 Dec 2022 08:27
    URI: http://repository.upi.edu/id/eprint/80530

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