PROFIL FISIKOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) ASAL SUMATERA UTARA

Tsara Ghaida Farlan, - (2019) PROFIL FISIKOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK N-HEKSAN BUAH ANDALIMAN (Zanthoxylum acanthopodium DC.) ASAL SUMATERA UTARA. S1 thesis, Universitas Pendidikan Indonesia.

Baca Full Text klik disini
[img] Text
S_KIM_1503847_Title.pdf
Download (571kB)
[img] Text
S_KIM_1503847_Chapter 1.pdf
Download (546kB)
[img] Text
S_KIM_1503847_Chapter 2.pdf
Restricted to Staf Perpustakaan
Download (952kB)
[img] Text
S_KIM_1503847_Chapter 3.pdf
Download (570kB)
[img] Text
S_KIM_1503847_Chapter 4.pdf
Restricted to Staf Perpustakaan
Download (725kB)
[img] Text
S_KIM_1503847_Chapter 5.pdf
Download (556kB)
[img] Text
S_KIM_1503847_Appendix.pdf
Restricted to Staf Perpustakaan
Download (1MB)
Official URL: http://repository.upi.edu

Abstract

Andaliman (Zanthoxylum acanthopodium DC.) dikenal sebagai salah satu bumbu masakan khas batak yang diketahui memiliki aktivitas antioksidan. Berdasarkan beberapa penelitian, tanaman andaliman mengandung beberapa metabolit sekunder antara lain alkaloid, flavonoid, steroid, fenolik, lignan, kumarin, terpenoid, dan tanin. Penelitian ini bertujuan untuk mengetahui karakterisasi fisikokimia dan aktivitas antioksidan dari ekstrak n-heksan buah andaliman. Karakterisasi fisikokimia dilakukan dengan kromatografi lapis tipis (KLT) fasa normal dan fasa terbalik, Fourier Transform Infra-Red (FTIR) serta uji aktivitas antioksidan dilakukan dengan metode peredaman radikal bebas DPPH (2,2-difenil-1-pikrilhidrazil). Maserasi 450 g buah andaliman segar menghasilkan ekstrak kental n-heksan 4,2694 g. Hasil analisis FTIR menunjukkan bahwa ekstrak memiliki gugus fungsi antara lain gugus O-H, C-H sp3, C=O, C=C aromatis, dan C-N, sehingga berdasarkan studi literatur diketahui bahwa ekstrak mengandung golongan senyawa fenolik dan non fenolik. Berdasarkan hasil analisis KLT fasa normal dan fasa terbalik memperlihatkan adanya tiga noda dengan nilai Rf berturut-turut sebesar 0,32; 0,60; dan 0,90 (n-heksan:etil asetat = 8:2) dan pada KLT fasa terbalik berturut-turut diperoleh nilai Rf sebesar 0,22; 0,35; dan 0,48 (metanol:air = 8:2). Hasil uji aktivitas antioksidan menunjukkan bahwa ekstrak n-heksan buah andaliman mempunyai nilai IC50 sebesar 849,56 ppm. Hasil tersebut menunjukkan bahwa ekstrak n-heksan buah andaliman sekurang-kurangnya memiliki tiga komponen senyawa dan memiliki aktivitas antioksidan. Andaliman (Zanthoxylum acanthopodium DC.) known as spice in Bataknese special herbs which is known to have antioxidant activity. Research on andaliman based on the literature contains several secondary metabolite such as alkaloids, flavonoids, steroids, phenolics, lignans, coumarins, terpenoids, and tannins. Aims of this study are to identify physicochemical characterization and antioxidant activity in n-hexane exctract of andaliman fruit. Physicochemical characterization was carried out using normal phase and refused phase thin layer chromatography (TLC), Fourier Transform Infra-Red (FTIR) analysis, and the antioxidant activity test was carried out by the DPPH free radical reduction method. 450 g of fresh andaliman fruits produces n-hexane 4,2694 g condensed extract with maseration process. Results of n-hexane extract of andaliman fruit analysis with FTIR showed that extract has OH functional group, CH sp3, C=O, C=C aromatics, and C-N, so based on literature studies it is known that the extract contains phenolic and non-phenolic compounds. Based on the results of the TLC analysis of the normal phase and refused phase showed the presence of three stains with Rf values of 0,32; 0,60; and 0,90 (n-hexane:ethyl acetate = 8:2) in normal phase and Rf values of 0,22; 0,35; and 0,48 (methanol:water) in refused phase. The analysis of antioxidant activity showed that n-hexane extract of andaliman fruit obtained IC50 value of 849,56 ppm. These results indicate that n-hexane extract of andaliman fruit has at least three component compounds and has antioxidant activity.

Item Type: Thesis (S1)
Subjects: L Education > L Education (General)
Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan)
Depositing User: Tsara Ghaida Farlan
Date Deposited: 06 Jul 2020 07:00
Last Modified: 06 Jul 2020 07:00
URI: http://repository.upi.edu/id/eprint/42387

Actions (login required)

View Item View Item