Alya Mardhotillah Azizah, - (2019) PENGARUH AIR TEBU SEBAGAI SUMBER KARBON ORGANIK TERHADAP PRODUKSI BIOMASSA DAN LIPID CHLORELLA VULGARIS DAN NAVICULA SALINICOLA. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Chlorella vulgaris dan Navicula salinicola merupakan kelompok mikroalga yang dilaporkan memiliki kandungan lipid yang cukup tinggi dan berpotensi untuk digunakan sebagai sumber alternatif penghasil senyawa prekursor bioenergi. Namun untuk mendapatkan suatu biomassa dan lipid yang tinggi dengan kondisi kultivasi autotropik masih menjadi suatu tantangan, sehingga kondisi kultivasi lain seperti fotoheterotropik perlu diamati. Selain itu, pengaruh kondisi kultivasi terhadap produksi biomassa dan lipid untuk mikroalga diatom khususnya Navicula salinicola masih kurang dieksplorasi. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan sumber karbon organik yaitu air tebu terhadap produksi biomassa dan lipid mikroalga Chlorella vulgaris dan Navicula salinicola. Analisis konsentrasi biomassa dilakukan dengan mengukur massa kering sel (Dry Cell Weight, DCW), konsentrasi lipid dilakukan dengan mengukur kadar total lipid dari hasil ekstraksi menggunakan metode Folch yang kemudian dihitung konsentrasi lipidnya berdasarkan kadar total lipid dan konsentrasi biomassa yang diperoleh, dan analisis asam lemak metil ester (Fatty Acid Methyl Ester, FAME) dilakukan dengan menggunakan GC-MS. Berdasarkan hasil penelitian ditunjukan bahwa penambahan air tebu sebagai sumber karbon organik terhadap produksi biomassa terjadi peningkatan biomassa pada Chlorella vulgaris dan Navicula salinicola sebesar 50,10% dan 69,40% dari konsentrasi biomassa yang dikultivasi tanpa penambahan air tebu. Demikian juga pengaruhnya terhadap produksi lipid terjadi peningkatan konsentrasi lipid pada Chlorella vulgaris dan Navicula salinicola sebesar 34,45% dan 25,51% dari konsentrasi lipid yang dikultivasi tanpa penambahan air tebu. Penambahan air tebu sebagai sumber karbon organik mengurangi variasi asam lemak pada Chlorella vulgaris dan Navicula salinicola sebanyak 3 dan 4 jenis dari mikroalga yang dikultivasi tanpa penambahan air tebu. Hal ini mengindikasikan penambahan air tebu dapat mensupresi variasi asam lemak pada Chlorella vulgaris ataupun Navicula salinicola tapi menstimulasi pembentukan asam palmitat (C16:0) yang berpotensi untuk diaplikasikan pada industri biodiesel. Chlorella vulgaris and Navicula salinicola are microalagae that has been reported to have high lipid content and have potential to be used as an alternative source of bioenergy precursor compounds. However, to obtain a high biomass and lipid with autotropic cultivation condition is still a challenge therefore other cultivation condition such as photoheterotropic need to be explored. In addition, the influence of cultivation conditions on the production of biomass and lipid for diatomic microalgae, especially Navicula salinicola, is still rare explored. This study aims to analyze the effect of organic carbon sources namely sugarcane addition on the production of biomass and lipid of Chlorella vulgaris and Navicula salinicola. Analysis of biomass concentrations is evaluated by measuring dry cell weight (DCW), while lipid concentrations is evaluated by measuring total lipid levels from the extraction results using the Folch method. Lipid concentrations is calculated based on total lipid levels and biomass concentration obtained. The profile of fatty acid methyl ester (FAME) is analysed by using GC-MS. Based on the results of this study, it was observed that addition of sugarcane as an organic carbon source has increased the biomass production in Chlorella vulgaris and Navicula salinicola by 50.10% and 69.40% respectively than those are cultivated without the addition of sugarcane. Similarlt, the addition of sugarcane has been showed to induced lipid accumulation in Chlorella vulgaris and Navicula salinicola by 34,45% and 25,51% respectively compared to those are cultivated with carbon source from the air. On the other hand, the addition of sugarcane as an organic carbon has decreased the variations of fatty acid in Chlorella vulgaris and Navicula salinicola by 3 and 4 types respectively than those are cultivated without the addition of sugarcane. The addition of sugarcane can suppress the variations of fatty acids in Chlorella vulgaris and Navicula salinicola but it stimulates the synthesis of palmitic acid (C16:0) which has potential to be applied to the biodiesel industry.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | No Panggil : S KIM ALY p-2019; Pembimbing : I. Gungun Gumilar, II. Heni Siti Halimatul; NIM : 1503649 |
| Uncontrolled Keywords: | Biomassa, Chlorella vulgaris,Fotoheterotropik, Lipid,Navicula salinicola |
| Subjects: | L Education > L Education (General) Q Science > QD Chemistry |
| Depositing User: | Alya Mardhotillah Azizah |
| Date Deposited: | 20 May 2020 02:50 |
| Last Modified: | 20 May 2020 02:50 |
| URI: | http://repository.upi.edu/id/eprint/42050 |
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