OPTIMASI DAN KARAKTERISASI NANOSRTUCTURED LIPID CARRIER L-DOPA-PALM OIL-LAURIC ACID (NLC-DPL) SEBAGAI KANDIDAT OBAT PARKINSON

Meliana Junita Azhari, - and Ratnaningsih Eko Sardjono, - and Vidia Afina Nuraini, - (2025) OPTIMASI DAN KARAKTERISASI NANOSRTUCTURED LIPID CARRIER L-DOPA-PALM OIL-LAURIC ACID (NLC-DPL) SEBAGAI KANDIDAT OBAT PARKINSON. S1 thesis, Universitas Pendidikan Indonesia.

Abstract

Parkinson merupakan penyakit neurodegeneratif yang ditandai dengan berkurangnya dopamin di otak. L-DOPA dipilih menjadi terapi utama untuk mengobati parkinson, namun memiliki keterbatasan bioavailabilitas dan stabilitas sehingga dilakukan enkapsulasi menggunakan sistem nanostructured lipid carrier (NLC). Penelitian ini bertujuan untuk memperoleh formulasi optimum, karakteristik, efisiensi pemuatan, pemuatan obat, serta profil pelepasan obat dari L-DOPA yang dienkapsulasi dengan NLC berbasis asam laurat dan minyak sawit (NLC-DPL). Metode pembuatan NLC-DPL menggunakan homogenisasi panas-ultrasonikasi dengan variabel optimasi meliputi perbandingan massa lipid, waktu ultrasonikasi, dan power rate ultrasonikasi. Karakterisasi NLC-DPL meliputi penentuan ukuran partikel, indeks polidispersitas (PI), dan zeta potensial menggunakan PSA, morfologi partikel menggunakan TEM dan SEM, serta gugus fungsi menggunakan FTIR. Penentuan efisiensi pemuatan, pemuatan obat, dan pelepasan obat menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan kondisi optimum pembuatan NLC-DPL diperoleh pada perbandingan massa asam laurat terhadap minyak sawit 2:8, waktu ulrasonikasi 60 menit, dan power rate 50%. Produk NLC-DPL yang diperoleh memiliki ukuran partikel sebesar 64,50 ± 0,17 dengan PI sebesar 0,217 serta zeta potensial sebesar -32,3 mV. Analisis FTIR menunjukkan pergeseran puncak gugus O-H yang mengindikasikan terjadinya interaksi antara L-DOPA dan matriks lipid. TEM menunjukkan ukuran partikel dengan kisaran 31,82–51,50 nm, sedangkan SEM menunjukkan morfologi spherical dengan komposisi unsur C (69,89%), N (11,12%), dan O (18,98%). Efisiensi pemuatan diperoleh sebesar 15,28% dengan pemuatan obat sebesar 0,182%. Profil pelepasan obat NLC-DPL menunjukkan pelepasan lambat dan terkontrol, yakni 9,02% pada pH = 1,2 dan 18,16% pada pH = 7,4 setelah 8 jam dan mengikuti model kinetika orde satu. Berdasarkan hasil tersebut, produk NLC-DPL memiliki potensi sebagai kandidat obat parkinson. Parkinson’s disease is a neurodegenerative disorder characterized by reduced dopamine levels in the brain. L-DOPA is chosen as the main therapy to treat Parkinson’s disease; however, it has limitations in bioavailability and stability, which is why encapsulation using the nanostructured lipid carrier (NLC) system is carried out. This study aimed to obtain the optimum formulation, characteristics, loading efficiency, drug loading, and drug release profile of L-DOPA encapsulated in lauric acid–palm oil–based NLC (NLC-DPL). The NLC-DPL was prepared using hot homogenization–ultrasonication with optimization variables including lipid ratio, ultrasonication time, and ultrasonication power rate. Characterization of NLC-DPL included particle size, polydispersity index (PI), and zeta potential using PSA, particle morphology using TEM and SEM, and functional groups using FTIR. Loading efficiency, drug loading, and drug release were determined using UV-Vis spectrophotometry. The results showed that the optimum conditions for the production of NLC-DPL were obtained at a mass ratio of lauric acid to palm oil of 2:8, an ultrasonication time of 60 minutes, and a power rate of 50%. The NLC-DPL product obtained had a particle size of 64.50 ± 0.17 with a PI of 0.217 and a zeta potential of -32.3 mV. FTIR analysis showed a shift in the O–H band, indicating interactions between L-DOPA and the lipid matrix. TEM revealed particle sizes ranging from 31.82 to 51.50 nm, while SEM demonstrated spherical morphology with elemental composition of C (69.89%), N (11.12%), and O (18.98%). The loading efficiency was 15.28% with drug loading of 0.182%. The drug release profile of NLC-DPL exhibited slow and controlled release, namely 9.02% at pH 1.2 and 18.16% at pH 7.4 after 8 hours, following first-order kinetics. Based on these results, NLC-DPL has potential as a candidate for Parkinson’s disease therapy.

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Item Type:	Thesis (S1)
Additional Information:	https://scholar.google.com/citations?view_op=new_profile&hl=id Ratnaningsih Eko Sardjono: 5979503 Vidia Afina Nuraini: 6792209
Uncontrolled Keywords:	L-DOPA, Nanostructured Lipid Carrier, Asam Laurat, Minyak Sawit, Parkinson L-DOPA, Nanostructured Lipid Carrier, Lauric Acid, Palm Oil, Parkinson’s disease
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan)
Depositing User:	Meliana Junita Azhari
Date Deposited:	16 Sep 2025 03:43
Last Modified:	16 Sep 2025 03:43
URI:	http://repository.upi.edu/id/eprint/139434

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