Yunita Rachmawati, - and Heli Siti Halimatul Munawaroh, - and Vidia Afina Nuraini, - (2025) KARAKTERISASI EKSTRAK DAN HIDROLISAT KOLAGEN DARI LIMBAH KULIT IKAN PATIN SERTA PREDIKSI POTENSI BIOAKTIVITASNYA SECARA IN SILICO SEBAGAI AGEN PENYEMBUH LUKA DIABETES. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Luka diabetes merupakan komplikasi diabetes melitus yang ditandai dengan penyembuhan luka yang lambat karena penurunan aktivitas angiogenesis. Angiogenesis berperan dalam regenerasi jaringan melalui pembentukan pembuluh darah baru. Industri pengolahan ikan patin menghasilkan limbah kulit yang dapat dimanfaatkan sebagai sumber kolagen alami untuk agen penyembuhan luka. Penelitian ini bertujuan mengevaluasi karakteristik ekstrak dan hidrolisat kolagen dari limbah kulit ikan patin serta memprediksi potensi bioaktivitasnya melalui studi in silico meliputi aktivitas pro-angiogenesis, antioksidan, dan antibakteri. Peptida aktif diperoleh melalui ekstraksi kolagen larut asam dan di karakterisasi dengan UV, FTIR, dan SDS-PAGE. Hidrolisat kolagen menggunakan enzim bromelin dan di karakterisasi menggunakan UV, FTIR, dan SDS-PAGE. Pengujian in vitro menunjukkan ekstrak kolagen mengandung protein 95,4% ± 2,00%, memiliki serapan UV khas kolagen pada 216 nm, gugus amida dan prolin/hidroksiprolin (FTIR), serta pita protein α (130 kDa) dan β (250 kDa) kolagen tipe I (SDS-PAGE). Hidrolisat kolagen menunjukkan derajat hidrolisis 63% pada 24 jam, hilangnya serapan kolagen pada 210–240 nm, pergeseran gugus (FTIR), serta pita <15 kDa (SDS-PAGE) yang menandakan fragmentasi menjadi peptida berukuran kecil. Hasil in silico menunjukkan bahwa peptida NF dan WKPEPCQICV memiliki afinitas ikatan yang tinggi terhadap VEGFR2 dan MMP-2 (pro-angiogenesis), KEAP1 (antioksidan), serta DNA Gyrase (antibakteri). Proses hidrolisis dapat memodifikasi struktur kolagen dan peptida aktif yang dihasilkan berpotensi dikembangkan sebagai agen terapi alami dalam merangsang pembentukan pembuluh darah baru, mengurangi stres oksidatif, dan menghambat infeksi bakteri pada luka diabetes. Validasi lanjutan secara in vitro terhadap bioaktivitas peptida ini direkomendasikan sebelum menuju tahap pengembangan aplikasi terapeutik. Diabetic wounds are a complication of diabetes mellitus characterized by slow wound healing due to decreased angiogenesis activity. Angiogenesis plays a role in tissue regeneration through the formation of new blood vessels. The catfish processing industry produces skin waste that can be utilized as a natural source of collagen for wound healing agents. This study aims to evaluate the characteristics of collagen extracts and hydrolysates from catfish skin waste and predict their potential bioactivity through in silico studies including pro-angiogenesis, antioxidant, and antibacterial activities. Active peptides were obtained through acid soluble collagen extraction and characterized by UV, FTIR, and SDS-PAGE. Collagen hydrolysate using bromelain enzyme and characterized using UV, FTIR, and SDS-PAGE. In vitro testing showed that the collagen extract contained 95.4% ± 2.00% protein, had typical collagen UV absorption at 216 nm, amide and proline/hydroxyproline groups (FTIR), and α (130 kDa) and β (250 kDa) protein bands of type I collagen (SDS-PAGE). The collagen hydrolysate showed 63% hydrolysis degree at 24 hours, loss of collagen absorption at 210-240 nm, cluster shift (FTIR), and <15 kDa band (SDS-PAGE) indicating fragmentation into small-sized peptides. In silico results showed that NF and WKPEPCQICV peptides have high binding affinity to VEGFR2 and MMP-2 (pro-angiogenesis), KEAP1 (antioxidant), and DNA Gyrase (antibacterial). The hydrolysis process can modify the structure of collagen and the resulting active peptides have the potential to be developed as natural therapeutic agents in stimulating the formation of new blood vessels, reducing oxidative stress, and inhibiting bacterial infection in diabetic wounds. Further in vitro validation of the bioactivity of these peptides is recommended before moving towards the development stage of therapeutic applications.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?hl=en&user=KR0dSUQAAAAJ ID SINTA Dosen Pembimbing Heli Siti Halimatul Munawaroh: 5978104 Vidia Afina Nuraini: 6792209 |
| Uncontrolled Keywords: | Peptida Aktif, Kulit Ikan Patin, Kolagen, Hidrolisat Kolagen, Luka Diabetes, In Silico, Angiogenesis. Active Peptides, Catfish Skin, Collagens, Collagen Hydrolysate, Diabetic Wounds, In Silico, Angiogenesis. |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan) |
| Depositing User: | Yunita Rachmawati |
| Date Deposited: | 12 Sep 2025 04:08 |
| Last Modified: | 12 Sep 2025 04:08 |
| URI: | http://repository.upi.edu/id/eprint/138708 |
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