OPTIMASI DAN KARAKTERISASI NANOSTRUCTURED LIPID CARRIER L-DOPA-PALM OIL-MYRISTIC ACID (NLC-DPM) SEBAGAI KANDIDAT OBAT PARKINSON

    Chyntia, Andies and Ratnaningsih Eko Sardjono, - and Vidia Afina Nuraini, - (2025) OPTIMASI DAN KARAKTERISASI NANOSTRUCTURED LIPID CARRIER L-DOPA-PALM OIL-MYRISTIC ACID (NLC-DPM) SEBAGAI KANDIDAT OBAT PARKINSON. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    L-DOPA adalah obat yang digunakan dalam pengobatan penyakit Parkinson tetapi memiliki keterbatasan dalam bioavailabilitas dan stabilitas. Penelitian ini bertujuan untuk mengembangkan sistem penghantaran obat yang berbasis Nanostructured Lipid Carrier (NLC) menggunakan lipid asam miristat dan minyak sawit (NLC-DPM) untuk meningkatkan karakteristik L-DOPA. NLC-DPM diformulasikan melalui metode homogenisasi panas dan ultrasonikasi dengan variabel optimasi seperti rasio lipid, konsentrasi surfaktan, durasi dan power rate ultrasonikasi. Karakterisasi meliputi ukuran partikel, indeks polidispersitas dan zeta potensial (PSA), morfologi (TEM dan SEM), gugus fungsi (FTIR), efisiensi enkapsulasi serta profil pelepasan obat (UV-Vis). Hasil penelitian menunjukkan bahwa kondisi optimum tercapai pada rasio lipid 1:9, dengan surfaktan 2%, serta waktu dan daya ultrasonikasi selama 80 menit dan 50%. NLC-DPM memiliki ukuran partikel 87,2±0,1, PI 0,114, dan zeta potensial -40,7 mV. Analisis FTIR menunjukkan adanya interaksi antara L-DOPA dan lipid pada gugus O-H, N-H, dan C=O, serta morfologi berbentuk bulat dengan ukuran 63.097 nm (TEM) dan dengan pemetaan EDS yaitu C (69.87%), N (7,82%) dan O (27,95%) yang menunjukkan bahwa L-DOPA sudah terperangkap dalam sistem NLC (SEM). Efisiensi enkapsulasi tercatat sebesar 51,36%, dengan profil pelepasan yang terkontrol hingga 8 jam, mencapai 19,45% pada pH 1,2 mengikuti kinetika pelepasan orde 1 dan 27,22% pada pH 7,4 mengikuti kinetika pelepasan orde 0. NLC-DPM memiliki potensi sebagai sistem penghantaran L-DOPA untuk terapi Parkinson. L-DOPA is a medication utilized in the treatment of Parkinson's disease; however, it has limitations regarding bioavailability and stability. This study aims to develop a drug delivery system based on Nanostructured Lipid Carrier (NLC) using myristic acid lipid and palm oil (NLC-DPM) to enhance the characteristics of L-DOPA. NLC-DPM is formulated through a hot homogenization and ultrasonication method, with optimization variables such as lipid ratio, surfactant concentration, duration, and power rate of ultrasonication. Characterization includes particle size, polydispersity index, and zeta potential (PSA), morphology (TEM and SEM), functional groups (FTIR), encapsulation efficiency, and drug release profile (UV-Vis). The research results indicate that optimal conditions are achieved at a lipid ratio of 1:9, with a surfactant concentration of 2%, and ultrasonication time and power of 80 minutes and 50%, respectively. NLC-DPM exhibits a particle size of 87.2±0.1, a PI of 0.114, and a zeta potential of -40.7 mV. FTIR analysis reveals interactions between L-DOPA and lipids at the O-H, N-H, and C=O groups, as well as a spherical morphology with a size of 63.097 nm (TEM) and EDS mapping showing C (69.87%), N (7,82%), and O (27,95%), indicating that L-DOPA is encapsulated within the NLC system (SEM). The encapsulation efficiency is recorded at 51.36%, with a controlled release profile over 8 hours, reaching 19.45% at pH 1.2 following first-order release kinetics and 27.22% at pH 7.4 following zero-order release kinetics. NLC-DPM shows potential as a delivery system for L-DOPA in Parkinson's therapy.

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    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: SINTA ID DOSEN PEMBIMBING Ratnaningsih Eko Sardjono: 5979503 Vidia Afina Nuraini: -
    Uncontrolled Keywords: L-DOPA, Parkinson, NCL, Asam Miristat, Minyak sawit, Formulasi
    Subjects: Q Science > QD Chemistry
    R Medicine > RS Pharmacy and materia medica
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan)
    Depositing User: Andies Chyntia
    Date Deposited: 17 Sep 2025 06:08
    Last Modified: 17 Sep 2025 06:08
    URI: http://repository.upi.edu/id/eprint/138649

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