ISOLASI DAN KARAKTERISASI SENYAWA FLAVONOID DARI KULIT BATANG Artocarpus elasticus SERTA UJI POTENSI SEBAGAI INHIBITOR ASETILKOLINESTERASE MELALUI PENGUJIAN IN SILICO

    Yolanda Sebastian, - and Iqbal Musthapa, - and Vidia Afina Nuraini, - (2025) ISOLASI DAN KARAKTERISASI SENYAWA FLAVONOID DARI KULIT BATANG Artocarpus elasticus SERTA UJI POTENSI SEBAGAI INHIBITOR ASETILKOLINESTERASE MELALUI PENGUJIAN IN SILICO. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Flavonoid merupakan metabolit sekunder yang telah dilaporkan memiliki beragam bioaktivitas. Flavonoid terprenilasi memiliki efek terapeutik pada penyakit degeneratif, termasuk penghambatan asetilkolinesterase (AChE). Jenis tumbuhan yang banyak menghasilkan flavonoid terprenilasi yaitu Artocarpus. Namun, flavonoid dari Artocarpus elasticus asal Indonesia belum pernah dilaporkan secara spesifik sebagai inhibitor AChE. Perbedaan habitat suatu tumbuhan dan faktor lingkungan dapat memengaruhi kandungan metabolit sekundernya. Penelitian ini bertujuan untuk menentukan struktur senyawa flavonoid dari kulit batang Artocarpus elasticus dan menguji aktivitasnya terhadap penghambatan AChE. Proses isolasi melalui tahap ekstraksi menggunakan metode maserasi, sedangkan tahap pemisahan dan pemurnian menggunakan kromatografi lapis tipis (KLT), kromatografi cair vakum (KCV), dan kromatografi kolom gravitasi (KKG). Senyawa hasil isolasi dikarakterisasi dengan spektroskopi Ultraviolet (UV), Fourier Transform Infra Red (FTIR), Nuclear Magnetic Resonance (NMR) dan spektroskopi massa (MS) serta diuji aktivitasnya terhadap penghambatan AChE secara in silico. Hasil penelitian diperoleh dua senyawa yaitu artobiloksanton (1) berupa padatan coklat kekuningan dan kudraflavon C (2) berupa padatan coklat yang telah dilaporkan sebelumnya. Uji in silico menunjukkan senyawa hasil isolasi memiliki aktivitas yang lemah sebagai inhibitor AChE dilihat dari nilai afinitas ikatan yang lebih tinggi (-4,1 kkal/mol (1) dan -3,9 kkal/mol (2)) dari donepezil (-12 kkal/mol) dan tidak adanya interaksi dengan residu kunci asam amino yang terlibat dalam penghambatan AChE. Flavonoids are secondary metabolites that have been reported to possess diverse bioactivities. Prenylated flavonoids have therapeutic effects in degenerative diseases, including acetylcholinesterase (AChE) inhibition. The type of plant that produces many prenylated flavonoids is Artocarpus. However, flavonoids from Artocarpus elasticus from Indonesia have never been reported specifically as AChE inhibitors. Differences in the habitat of a plant and environmental factors can affect the content of its secondary metabolites. The purpose of this study was to determine the structure of flavonoid compounds from the stem bark of Artocarpus elasticus and test their activity towards AChE inhibition. The isolation process involved extraction using maceration method, while the separation and purification used thin layer chromatography (TLC), vacuum liquid chromatography (VLC), and gravity column chromatography (GCG). The isolated compounds were characterised by Ultraviolet (UV) spectroscopy, Fourier Transform Infra Red (FTIR), Nuclear Magnetic Resonance (NMR) and mass spectroscopy (MS) and tested for their activity against AChE inhibition in silico. The results obtained two compounds, namely artobiloxanthone (1) in the form of yellowish brown solids and cudraflavone C (2) in the form of brown solids that have been reported previously. In silico tests showed that the isolated compounds have weak activity as AChE inhibitors, as seen from the higher binding affinity (-4.1 kcal/mol (1) and -3.9 kcal/mol (2)) than donepezil (-12 kcal/mol), and no interaction with key amino acid residues involved in AChE inhibition.

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    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?view_op=new_articles&hl=en&imq=Yolanda+Sebastian# ID SINTA Dosen Pembimbing: Iqbal Musthapa: 5979687 Vidia Afina Nuraini: 6792209
    Uncontrolled Keywords: Artocarpus elasticus, flavonoid, artobiloksanton, kudraflavon C, asetilkolinesterase Artocarpus elasticus, flavonoids, artobiloxanthone, cudraflavone C, acetylcholinesterase
    Subjects: L Education > L Education (General)
    Q Science > QD Chemistry
    R Medicine > RS Pharmacy and materia medica
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan)
    Depositing User: Yolanda Sebastian
    Date Deposited: 04 Sep 2025 15:51
    Last Modified: 04 Sep 2025 15:51
    URI: http://repository.upi.edu/id/eprint/137473

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