ISOLASI DAN KARAKTERISASI FLAVONOID TERISOPRENILASI DARI AKAR Artocarpus heterophyllus SEBAGAI INHIBITOR ASETILKOLINESTERASE: Pengujian Potensi In Silico

    Laras Adistya Putri, - and Iqbal Musthapa, - and Vidia Afina Nuraini, - (2025) ISOLASI DAN KARAKTERISASI FLAVONOID TERISOPRENILASI DARI AKAR Artocarpus heterophyllus SEBAGAI INHIBITOR ASETILKOLINESTERASE: Pengujian Potensi In Silico. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Tumbuhan Artocarpus heterophyllus, atau nangka, merupakan tumbuhan yang memiliki berbagai senyawa dengan potensi biologis seperti flavonoid, prenilflavonoid, sterol, stilbenoid, polifenol, dan terpenoid yang telah dilaporkan memiliki aktivitas antioksidan, antiinflamasi, antibakteri, antikanker, antikariogenik, dan antijamur. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi senyawa turunan flavonoid dari akar tumbuhan nangka, serta menguji aktivitas antialzheimer secara in silico. Tahap isolasi dilakukan dengan ekstraksi, fraksinasi, dan pemurnian menggunakan berbagai teknik kromatografi, yaitu Kromatografi Cair Vakum (KCV), Kromatografi Kolom Gravitasi (KKG), dan Kromatografi Lapis Tipis (KLT). Dari tahap pemisahan dan pemurnian, dihasilkan 1 isolat berupa padatan coklat kemerahan sebanyak 29,7 mg. Karakterisasi struktur senyawa yang diperoleh didasarkan pada data spektroskopi Nuclear Magnetic Resonance (NMR) 1D (1H-NMR) dan membandingkannya dengan literatur. Hasil dari karakterisasi tersebut menunjukkan senyawa isolat 1 memiliki struktur yang serupa dengan senyawa Licoflavone C. Uji aktivitas secara in silico dengan aplikasi BIOVIA Discovery Studio Visualizer dan AutoDock Tools menunjukkan bahwa senyawa licoflavone C memiliki aktivitas yang lemah untuk dijadikan sebagai kandidat obat antialzheimer dalam menggantikan donepezil melalui mekanisme penghambatan asetilkolinesterase. Artocarpus heterophyllus, or jackfruit, is a plant that has various compounds with biological potential such as flavonoids, prenylflavonoids, sterols, stilbenoids, polyphenols, and terpenoids that have been reported to have antioxidant, anti-inflammatory, antibacterial, anticancer, anticariogenic, and antifungal activities. This research aims to isolate and characterize flavonoid-derived compounds from jackfruit roots, as well as to test their anti-Alzheimer activity with in silico method. The isolation stage was carried out by extraction, fractionation, and purification using various chromatographic techniques, such as Vacuum Liquid Chromatography (VLC), Gravity Column Chromatography (GCC), and Thin Layer Chromatography (TLC). From the separation and purification stages, isolate 1 was produced in the form of a reddish-brown solid was obtained, weighing 29.7 mg. The structural characterization of the compound obtained was based on 1D Nuclear Magnetic Resonance (NMR) spectroscopy data (1H-NMR) and comparing it with the literature. The result of the characterization showed that isolate 1 compound has a similar structure to the Licoflavone C compound. In silico activity test using the BIOVIA Discovery Studio Visualizer and AutoDock Tools applications showed that the licoflavone C compound has weak activity to be used as a candidate of anti-Alzheimer's drug to replace donepezil through the acetylcholinesterase inhibition mechanism.

    Baca Full Text klik disini
    [thumbnail of S_KIM_2103259_Title.pdf] Text
    S_KIM_2103259_Title.pdf
    Download (481kB)
    [thumbnail of S_KIM_2103259_Chapter1.pdf] Text
    S_KIM_2103259_Chapter1.pdf
    Download (269kB)
    [thumbnail of S_KIM_2103259_Chapter2.pdf] Text
    S_KIM_2103259_Chapter2.pdf
    Restricted to Staf Perpustakaan
    Download (1MB)
    [thumbnail of S_KIM_2103259_Chapter3.pdf] Text
    S_KIM_2103259_Chapter3.pdf
    Download (311kB)
    [thumbnail of S_KIM_2103259_Chapter4.pdf] Text
    S_KIM_2103259_Chapter4.pdf
    Restricted to Staf Perpustakaan
    Download (823kB)
    [thumbnail of S_KIM_2103259_Chapter5.pdf] Text
    S_KIM_2103259_Chapter5.pdf
    Download (251kB)
    [thumbnail of S_KIM_2103259_Appendix.pdf] Text
    S_KIM_2103259_Appendix.pdf
    Restricted to Staf Perpustakaan
    Download (534kB)
    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?view_op=list_works&hl=en&user=gvJCRJ4AAAAJ ID SINTA Dosen Pembimbing: Iqbal Musthapa: 0023127503 Vidia Afina Nuraini: 0005079302
    Uncontrolled Keywords: Akar nangka, alzheimer, asetilkolinesterase, flavonoid, licoflavone C Acetylcholinesterase, Alzheimer's disease, flavonoids, jackfruit’s root, licoflavone C
    Subjects: Q Science > QD Chemistry
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan)
    Depositing User: Laras Adistya Putri
    Date Deposited: 16 Sep 2025 01:32
    Last Modified: 16 Sep 2025 01:32
    URI: http://repository.upi.edu/id/eprint/137212

    Actions (login required)

    View Item View Item