KANDUNGAN METABOLIT DAN AKTIVITAS ANTIOKSIDAN PADA TINGKAT PERKEMBANGAN DAUN CEMARA SUMATRA (Taxus sumatrana (Miq.) de Laub

    Emilia Mia Lestari, - and R Kusdianti, - and Any Fitriani, - (2025) KANDUNGAN METABOLIT DAN AKTIVITAS ANTIOKSIDAN PADA TINGKAT PERKEMBANGAN DAUN CEMARA SUMATRA (Taxus sumatrana (Miq.) de Laub. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Tanaman Genus Taxus telah dikenal memiliki potensi antikanker sejak tahun 1972 yang pertama kali diidentifikasi dari ekstrak Taxus brevifolia Nutt di Amerika Serikat. Di Indonesia, satu-satunya spesies yang termasuk Genus ini adalah Taxus sumatrana. Penelitian terdahulu menemukan senyawa antikanker pada kulit batang dan batang muda. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit dan aktivitas antioksidan pada dua tingkat perkembangan daun T. sumatrana, yaitu daun dewasa dan daun tua. Daun diambil dari Kebun Raya Cibodas, Kabupaten Cianjur, dari satu bagian batang yang sama pada lima individu berdasarkan keseragaman morfologi. Daun dikeringkan, dihaluskan, dan diekstrak dengan metode maserasi menggunakan pelarut etanol 70% p.a. Kandungan metabolit pada daun dianalisis menggunakan instrumen Gas Chromatography-Mass Spectrometry (GC-MS) dan diidentifikasi berdasarkan indeks kesamaannya dengan pustaka National Institute of Standards Technology (NIST))dan PubChem. Aktivitas antioksidan dianalisis menggunakan metode DPPH dengan pelarut metanol menggunakan spektrometer pada panjang gelombang 515 nm. Hasil penelitian menunjukkan daun dewasa mengandung 9 senyawa, sedangkan daun tua mengandung 13 senyawa. Kedua tingkat daun mengandung 4 senyawa yang sama dan didominasi oleh senyawa metil palmitat. Hasil uji aktivitas antioksidan pada daun dewasa sebesar 13,82 ppm dan daun tua sebesar 20,95 ppm yang termasuk dalam kategori sangat kuat. Penelitian ini menunjukkan adanya perbedaan kandungan metabolit pada tingkat perkembangan daun T. sumatrana, dan aktivitas antioksidan ekstrak daun dewasa lebih tinggi dibanding ekstrak daun tua. The Taxus genus has been recognized for its anticancer potential since 1972, first identified from Taxus brevifolia Nutt extract in the United States. In Indonesia, the only species belonging to this genus is Taxus sumatrana. Previous studies have found anticancer compounds in the bark and young stems. This study aims to identify the metabolite content and antioxidant activity in two developmental stages of T. sumatrana leaves, namely mature and senescent leaves. Leaves were collected from the Cibodas Botanical Garden, Cianjur Regency, from the same branch section on five individuals selected for morphological uniformity. The leaves were dried, ground, and extracted by maceration using 70% ethanol (p.a.) as the solvent. Metabolite contents were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and identified based on their similarity index with the National Institute of Standards and Technology (NIST) and PubChem libraries. Antioxidant activity was analyzed using the DPPH method with methanol as a solvent and measured by spectrophotometry at a wavelength of 515 nm. The results showed that mature leaves contained 9 compounds, while senescent leaves contained 13 compounds. Both leaf stages shared 4 common compounds and were dominated by methyl palmitate. Antioxidant activity tests showed values of 13.82 ppm for mature leaves and 20.95 ppm for senescent leaves, both classified as very strong. This study demonstrates differences in metabolite content across T. sumatrana leaf development stages, with mature leaf extracts exhibiting higher antioxidant activity than senescent leaf extracts.

    Baca Full Text klik disini
    [thumbnail of S_BIO_2109093_Title.pdf] Text
    S_BIO_2109093_Title.pdf
    Download (562kB)
    [thumbnail of S_BIO_2109093_Chapter1.pdf] Text
    S_BIO_2109093_Chapter1.pdf
    Download (140kB)
    [thumbnail of S_BIO_2109093_Chapter2.pdf] Text
    S_BIO_2109093_Chapter2.pdf
    Restricted to Staf Perpustakaan
    Download (374kB)
    [thumbnail of S_BIO_2109093_Chapter3.pdf] Text
    S_BIO_2109093_Chapter3.pdf
    Download (815kB)
    [thumbnail of S_BIO_2109093_Chapter4.pdf] Text
    S_BIO_2109093_Chapter4.pdf
    Restricted to Staf Perpustakaan
    Download (653kB)
    [thumbnail of S_BIO_2109093_Chapter5.pdf] Text
    S_BIO_2109093_Chapter5.pdf
    Download (41kB)
    [thumbnail of S_BIO_2109093_Appendix.pdf] Text
    S_BIO_2109093_Appendix.pdf
    Restricted to Staf Perpustakaan
    Download (250kB)
    Official URL: https://repository.upi.edu
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?view_op=new_profile&hl=en ID SINTA Dosen Pembimbing: R Kusdianti: 6041647 Any Fitriani: 6004273
    Uncontrolled Keywords: Daun T. sumatrana, Gas Chromatography-Mass Spectrometry (GC-MS), metabolit, antioksidan, tingkat perkembangan. T. sumatrana leaves, Gas Chromatography-Mass Spectrometry (GC-MS), metabolites, antioxidants, developmental stage.
    Subjects: L Education > L Education (General)
    R Medicine > R Medicine (General)
    R Medicine > RS Pharmacy and materia medica
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
    Depositing User: Emilia Mia Lestari
    Date Deposited: 02 Sep 2025 06:46
    Last Modified: 02 Sep 2025 06:46
    URI: http://repository.upi.edu/id/eprint/137121

    Actions (login required)

    View Item View Item