BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA KONSORSIUM BAKTERI BACILLUS LICHENIFORMIS, BACILLUS PARALICHENIFORMIS, DAN BREVIBACILLUS BORSTELENSIS SECARA IN VITRO

    Ok Muhammad Abthal Al Wafi, - and Wahyu Surakusumah, - and Diah Kusumawaty, - (2025) BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA KONSORSIUM BAKTERI BACILLUS LICHENIFORMIS, BACILLUS PARALICHENIFORMIS, DAN BREVIBACILLUS BORSTELENSIS SECARA IN VITRO. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Industri penyamakan kulit umumnya menggunakan chromosal B sebagai bahan penyamak kulit yang mengandung logam krom, namun sebagian krom akan terbuang sebagai limbah yang mencemari lingkungan. Salah satu metode penanganannya adalah bioremediasi, di mana penggunaan konsorsium bakteri terbukti lebih efektif dibandingkan isolat tunggal. Penelitian ini bertujuan menentukan kombinasi spesies bakteri paling optimal dalam formula konsorsium sebagai agen bioremediasi logam krom. Bakteri diisolasi dari tanah rizosfer tumbuhan di sekitar bak limbah penyamakan wilayah Sukaregang, Kabupaten Garut. Seleksi dilakukan terhadap isolat yang menunjukkan resistensi tertinggi terhadap krom. Isolat terpilih kemudian diidentifikasi melalui uji morfologi, biokimia, dan analisis molekuler berbasis gen 16S rRNA. Berdasarkan analisis tersebut, diperoleh tiga spesies bakteri: Bacillus licheniformis, Bacillus paralicheniformis, dan Brevibacillus borstelensis. Konsorsium dibentuk berdasarkan uji kompatibilitas dan laju pertumbuhan yang menunjukkan interaksi sinergis antar spesies tanpa dominansi atau antagonisme. Uji efisiensi bioremoval menunjukkan bahwa kombinasi ketiga spesies menghasilkan efisiensi tertinggi sebesar 32,71% dalam inkubasi 14 jam. Spesies B. licheniformis dan B. paralicheniformis berperan dominan dalam proses bioremoval, sementara semua bakteri dalam konsorsium mampu bertahan dalam kondisi toksik logam krom. Temuan ini diharapkan dapat menjadi referensi ilmiah dalam mendukung pengembangan teknologi bioremediasi yang lebih spesifik dan aplikatif untuk menangani pencemaran logam krom, serta menjadi dasar pemanfaatan bakteri resisten sebagai agen bioremediasi pada limbah industri penyamakan kulit. The leather tanning industry commonly uses chromosal B as a tanning agent containing chromium; however, part of this chromium is discharged as waste, polluting the environment. One remediation strategy is bioremediation, in which bacterial consortia have proven more effective than single isolates. This study aimed to determine the optimal combination of bacterial species in a consortium formulation for chromium bioremediation. Bacteria were isolated from rhizosphere soil near tanning wastewater ponds in Sukaregang, Garut Regency. Selection was based on isolates exhibiting the highest chromium resistance. Selected isolates were identified through morphological and biochemical tests, as well as 16S rRNA gene-based molecular analysis. Three species were obtained: Bacillus licheniformis, Bacillus paralicheniformis, and Brevibacillus borstelensis. Consortium formulation was guided by compatibility tests and growth rate assessments, revealing synergistic interactions without dominance or antagonism. Bioremoval tests showed that the three-species combination achieved the highest efficiency of 32.71% after 14 hours of incubation. B. licheniformis and B. paralicheniformis played dominant roles, while all species tolerated chromium toxicity with varying contributions. These findings may serve as a scientific reference for developing targeted and applicable bioremediation technologies to address heavy metal pollution. Furthermore, the study underscores the potential of metal-resistant bacteria as effective bioremediation agents and highlights the value of biochemical and molecular identification in assessing bacterial potential for treating industrial wastewater, particularly chromium-containing leather tanning effluent.

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    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?view_op=new_profile&hl=en&authuser=2 ID SINTA Dosen Pembimbing: Wahyu Surakusumah: 6723267 Diah Kusumawaty: 6005459
    Uncontrolled Keywords: Bioremedisi, Konsorsium bakteri, Limbah Logam Krom, 16S rRNA, Efisiensi Bioremoval. Bioremediation, Bacterial consortium, Chromium Waste, 16S rRNA, Bioremoval Efficiency.
    Subjects: Q Science > QR Microbiology
    T Technology > TD Environmental technology. Sanitary engineering
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
    Depositing User: Ok Muhammad Abthal Al Wafi
    Date Deposited: 02 Sep 2025 08:20
    Last Modified: 02 Sep 2025 08:20
    URI: http://repository.upi.edu/id/eprint/136852

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