AKTIVITAS ANTIINFLAMASI DAN POTENSI ANTIKANKER FORMULASI NANOKRISTAL EKSTRAK DAUN KELOR DAN CIPLUKAN SECARA IN VITRO

Sekar Putri Firlianti, - and Didik Priyandoko, - and R. Kusdianti, - (2025) AKTIVITAS ANTIINFLAMASI DAN POTENSI ANTIKANKER FORMULASI NANOKRISTAL EKSTRAK DAUN KELOR DAN CIPLUKAN SECARA IN VITRO. S1 thesis, Universitas Pendidikan Indonesia.

Abstract

Stres oksidatif memicu inflamasi kronis yang dapat menyebabkan kerusakan DNA dan perkembangan kanker. Pengobatan konvensional sering menimbulkan efek samping seperti gangguan pencernaan, resistensi obat, dan toksisitas sistemik. Penelitian ini bertujuan mengevaluasi potensi kombinasi nanokristal ekstrak daun kelor dan ciplukan (NKEKC) sebagai agen antioksidan, antiinflamasi, dan antikanker secara in vitro. Nanokristal dibuat dari ekstrak serbuk kelor dan ciplukan, kemudian dikarakterisasi PSA dan ZPA. Hasil menunjukkan ukuran partikel NKEK dan NKEC masing-masing 323 nm dan 671 nm, dengan zeta potensial -40.5 mV dan -52.3 mV. Uji DPPH menunjukkan NKEKC mampu menghambat radikal bebas hingga 55,06% pada konsentrasi 4 mg/mL. Pada uji denaturasi protein, NKEKC menghambat denaturasi hingga 98,39% pada konsentrasi 1 mg/mL. Uji sitotoksisitas pada sel MCF-7 menunjukkan viabilitas sel 87,48% pada konsentrasi 1 mg/mL, namun belum mencapai IC50. Selain itu, NKEKC mampu menginduksi penuaan sel (senescence) pada sel MCF-7 sebesar 53,47% pada konsentrasi 0,25 mg/mL dan meningkat hingga 87,78% pada 4 mg/mL. Analisis siklus sel NKEKC terhadap sel MCF-7 menunjukkan bahwa NKEKC dapat menginduksi penghentian siklus sel pada fase G0 dan S. Berdasarkan hasil tersebut, dapat disimpulkan bahwa ekstrak kelor dan ekstrak ciplukan tergolong partikel nano yang memiliki aktivitas antioksidan dan antiinflamasi yang baik. NKEKC berpotensi sebagai agen antikanker melalui mekanisme induksi penuaan sel. Oxidative stres triggers chronic inflammation, which can lead to DNA damage and cancer development. Conventional treatments often cause side effects such as digestive disorders, drug resistance, and systemic toxicity. This study aims to evaluate the potential of a combination of nanocrystals from Moringa oleifera and Physalis angulata leaf extracts (NKEKC) as antioxidant, anti-inflammatory, and anticancer agents in vitro. Nanocrystals were prepared from powdered extracts of Moringa and Physalis and characterized using particle size analysis and zeta potential analysis. The results showed that the particle sizes of NKEK and NKEC were 323 nm and 671 nm, respectively, with zeta potentials of -40.5 mV and -52.3 mV. NKEKC showed a particle size of 1115 nm and a zeta potential of -22.2 mV. The DPPH assay indicated that NKEKC could inhibit free radicals up to 55.06% at a concentration of 4 mg/mL. In the protein denaturation assay, NKEKC inhibited protein denaturation by 98.39% at 1 mg/mL. Cytotoxicity testing on MCF-7 cells showed a cell viability of 87.48% at 1 mg/mL, indicating that NKEKC has not yet reached the IC50 value. Moreover, NKEKC induced cellular senescence in MCF-7 cells by 53.47% at 0.25 mg/mL, increasing to 87.78% at 4 mg/mL. NKEKC cell cycle analysis of MCF-7 cells showed that NKEKC can induce cell cycle arrest in the G0 and S phases. Based on these results, it can be concluded that Moringa and Physalis extract are classified as nanocrystals with good antioxidant and anti-inflammatory activity. NKEKC also has potential as an anticancer agent through the induction of cellular senescence.

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Item Type:	Thesis (S1)
Additional Information:	https://scholar.google.com/citations?user=LWdgSZgAAAAJ&hl=id ID SINTA Dosen Pembimbing: Didik Priyandoko: 6658318 R Kusdianti: 6041647
Uncontrolled Keywords:	stres oksidatif, inflamasi, kanker, kelor, ciplukan, nanokristal oxidative stress, inflammation, cancer, kelor, ciplukan, nanocrystal
Subjects:	R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RV Botanic, Thomsonian, and eclectic medicine
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
Depositing User:	Sekar Putri Firlianti
Date Deposited:	02 Sep 2025 03:38
Last Modified:	02 Sep 2025 03:38
URI:	http://repository.upi.edu/id/eprint/136746

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