BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA DARI KONSORSIUM BAKTERI Brevibacillus borstelensis, Bacillus licheniformis, DAN Bacillus sp.

    Salma Setia Nurhaliza, - and Wahyu Surakusumah, - and Any Aryani, - (2025) BIOREMEDIASI LOGAM KROMIUM MENGGUNAKAN KOMBINASI FORMULA DARI KONSORSIUM BAKTERI Brevibacillus borstelensis, Bacillus licheniformis, DAN Bacillus sp. S1 thesis, Universitas Pendidikan Indonesia.

    Abstract

    Logam kromium merupakan salah satu polutan prioritas karena menimbulkan berbagai resiko bagi organisme, mikroorganisme, dan lingkungan. Industri kerajinan kulit menjadi salah satu penghasil limbah yang mengandung logam kromium dari limbah penyamakan kulit. Upaya yang dapat dilakukan untuk mengurangi pencemaran logam kromium di lingkungan yaitu dengan metode bioremediasi. Bioremediasi dapat menggunakan mikroorganisme yaitu bakteri, penggunaan konsorsium bakteri dianggap lebih efektif dibandingkan dengan bakteri tunggal. Penelitian ini bertujuan untuk menganalisis kemampuan formula konsorsium bakteri yang berpotensi sebagai agen bioremediasi logam kromium secara in vitro. Bakteri diisolasi dari tanah area rhizosfer tumbuhan yang berada di sekitar bak penampungan limbah di wilayah Sukaregang, Kabupaten Garut. Bakteri diseleksi dengan memilih bakteri yang memiliki resistensi tertinggi lalu diidentifikasi berdasarkan aktivitas biokimia dan gen 16S rRNA. Kemampuan bioremediasi logam kromium ditunjukkan berdasarkan interaksi antar bakteri dan persentase pengurangan Cr(VI) oleh konsorsium bakteri. Identifikasi bakteri berdasarkan aktivitas biokimia yang dilengkapi dengan analisis gen 16S rRNA menunjukkan ketiga isolat bakteri teridentifikasi sebagai Brevibacillus borstelensis, Bacillus licheniformis, dan Bacillus sp.. Hasil uji interaksi antar bakteri menunjukkan bahwa bakteri penyusun konsorsium saling bersinergi. Seluruh konsorsium bakteri mampu mengurangi Cr(VI) namun memiliki kemampuan yang berbeda. Konsorsium dengan kombinasi tiga bakteri yaitu Brevibacillus borstelensis, Bacillus licheniformis, dan Bacillus sp. memiliki persentase pengurangan Cr(VI) tertinggi yaitu sebesar 39,38%. Chromium metal is one of the priority pollutants because it poses various risks to organisms, microorganisms, and the environment. The leather craft industry is one of the producers of waste containing chromium metal from tanning waste. Efforts that can be made to reduce chromium metal pollution in the environment are by bioremediation methods. Bioremediation can use microorganisms, namely bacteria, the use of bacterial consortia is considered more effective than single bacteria. This study aims to analyze the ability of bacterial consortium formulas that have the potential as chromium metal bioremediation agents in vitro. Bacteria were isolated from the soil of the rhizosphere area of plants around the waste storage tank in the Sukaregang area, Garut Regency. Bacteria were selected by selecting bacteria that had the highest resistance and then identified based on biochemical activity and the 16S rRNA gene. The ability of chromium metal bioremediation was shown based on the interaction between bacteria and the percentage of Cr(VI) reduction by the bacterial consortium. Identification of bacteria based on biochemical activity supported by 16S rRNA gene analysis showed that the three bacterial isolates were identified as Brevibacillus borstelensis, Bacillus licheniformis, and Bacillus sp.. The results of the interaction test between bacteria showed that the bacteria that make up the consortium synergize with each other. All bacterial consortia are able to reduce Cr(VI) but have different abilities. The consortium with a combination of three bacteria, namely Brevibacillus borstelensis, Bacillus licheniformis, and Bacillus sp. has the highest Cr(VI) reduction percentage of 39.38%.

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    Official URL: https://repository.upi.edu/
    Item Type: Thesis (S1)
    Additional Information: https://scholar.google.com/citations?view_op=new_articles&hl=en&imq=Salma+Setia+Nurhaliza# ID SINTA Dosen Pembimbing: Wahyu Surakusumah: 6723267 Any Aryani: 6038233
    Uncontrolled Keywords: Bioremediasi, Gen 16S rRNA, Konsorsium, Kromium, Penyamakan Kulit Bioremediation, 16S rRNA Gene, Consortium, Chromium, Leather Tanning
    Subjects: Q Science > QH Natural history > QH301 Biology
    Q Science > QR Microbiology
    Divisions: Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan)
    Depositing User: Salma Setia Nurhaliza
    Date Deposited: 20 Aug 2025 02:37
    Last Modified: 20 Aug 2025 02:37
    URI: http://repository.upi.edu/id/eprint/135877

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