Ai Susanti, - (2024) OPTIMASI DAN KARAKTERISASI NANOSTRUCTURED LIPID CARRIER DARI L-DOPA-ASAM LAURAT-MINYAK KEDELAI (NLC-DSL) SEBAGAI KANDIDAT OBAT PARKINSON. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
L-Dopa menjadi pilihan utama dalam pengobatan penyakit parkinson yang mempunyai keterbatasan bioavaibilitas dan stabilitas sehingga diperlukan enkapsulasi menggunakan sistem Nanostructured Lipid Carrier (NLC). Penelitian ini bertujuan untuk mendapatkan formulasi optimum, karakteristik, nilai entrapment efficiency dan profil drug release dari produk L-Dopa berbasis asam laurat dan minyak kedelai (NLC-DSL). Pembuatan NLC-DSL dilakukan menggunakan homogenisasi panas dan ultrasonikasi dengan variabel optimasi meliputi perbandingan lipid, konsentrasi surfaktan, dan waktu ultrasonikasi. Karakterisasi NLC-DSL meliputi penentuan ukuran partikel, polydispersity indeks, dan zeta potensial menggunakan PSA, morfologi menggunakan TEM, gugus fungsi menggunakan FTIR. Penentuan nilai entrapment efficiency dan profil drug release dilakukan menggunakan spektrofotometri UV-Vis. Hasil penelitian menunjukkan kondisi optimum pembuatan NLC-DSL diperoleh pada perbandingan asam laurat terhadap minyak kedelai 1:9, konsentrasi surfaktan 2,5% dan waktu ultrasonikasi 40 menit. Produk NLC-DSL yang diperoleh memiliki ukuran partikel 49,4±0,07 nm dengan polydispersity indeks 0,451 serta zeta potensial -44,3 mV. Analisis dengan FTIR menunjukkan adanya pergeseran puncak serapan gugus C=O, O-H dan N-H yang mengidentifikasikan adanya interaksi antara senyawa L-Dopa dengan asam laurat dan minyak kedelai. Sementara itu hasil TEM menunjukkan bentuk spherical dengan ukuran kisaran 49,73 nm. Persentase entrapment efficiency dari NLC-DSL diperoleh sebesar 78,05% dan profil drug release NLC-DSL menunjukkan kemampuan melepaskan L-Dopa secara lambat dan terkontrol hingga 6 jam dan mencapai 51,20% pada pH 1,2 dengan mengikuti model kinetika korsmeyer-peppas dan 72,87% pada pH 7,4 dengan mengikuti model kinetika orde nol setelah 24 jam. Berdasarkan hasil yang diperoleh, produk NLC-DSL memiliki potensi sebagai kandidat obat parkinson. L-Dopa is the primary choice in the treatment of Parkinson's disease, which has limited bioavailability and stability, requiring encapsulation using the nanostructured lipid carrier system (NLC). The research aims to obtain optimum formulation, characteristics, entrapment efficiency values, and drug release profiles of L-Dopa lauric acid and soy oil-based products (NLC-DSL). The production of NLC-DSL is done using heat homogenization and ultrasonication with optimization variables covering lipid ratio, surface agent concentration, and ultrasound time. NLC-DSL characterization includes particle size determination, polydispersity index, and zeta potential using PSA, morphology using TEM, and function clustering using FTIR. The determination of entrapment efficiency values and drug release profile is done using UV-Vis spectroscopic photometry. The results show the optimum condition of production of NLC-DSL is obtained at a comparison of lauric acid to soy oil 1:9, a surface agent concentration of 2.5%, and an ultrasound time of 40 minutes. The resulting NLC-DSL product has a particulate size of 49,4±0,07 nm with a polidispersibility index of 0,451 and a potential zeta of -44,3 mV. Analysis with FTIR shows a peak shift in the cluster of C=O, O-H and N-H that identifies the interaction between L-Dopa compounds with lauric acid and soybean oil. Meanwhile, TEM results showed a spherical shape with an average size of 49.73 nm. The percentage entrapment efficiency of NLC-DSL was obtained at 78.05%, and the NLC-DSL drug release profile showed the ability to release L-Dopa slowly and controlled for up to 6 hours and reach 51.20% at pH 1.2 following the Korsmeyer-Peppas kinetic model and 72.87% at pH 7.4 following the zero-order kinetics model after 24 hours. Based on the results obtained, the NLC-DSL product has potential as a candidate drug for Parkinson's disease.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?user=O40bYSAAAAAJ&hl=id&authuser=1 SINTA ID Ratnaningsih Eko Sardjono: 5979503 SINTA ID Vidia Afina Nuraini: 6792209 |
| Uncontrolled Keywords: | L-Dopa, Nanostructured Lipid Carrier¸ Asam Laurat, Minyak Kedelai, Parkinson Lauric Acid, Soybean Oil, Parkinson’s disease. |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan) |
| Depositing User: | Ai Susanti |
| Date Deposited: | 17 Sep 2024 02:01 |
| Last Modified: | 18 Sep 2024 02:48 |
| URI: | http://repository.upi.edu/id/eprint/125098 |
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