KARAKTERISASI DAN UJI AKTIVITAS RAFINAT FUKOIDAN DARI Sargassum polycystum SEBAGAI INHIBITOR ENZIM α-AMILASE

Alya Nurhidayati, - (2024) KARAKTERISASI DAN UJI AKTIVITAS RAFINAT FUKOIDAN DARI Sargassum polycystum SEBAGAI INHIBITOR ENZIM α-AMILASE. S1 thesis, Universitas Pendidikan Indonesia.

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Abstract

Diabetes melitus tipe-2 (T2DM) merupakan penyakit metabolik kronik yang ditandai dengan tingginya kadar gula dalam darah. Inhibisi enzim α-amilase dapat membantu memperlambat hidrolisis karbohidrat menjadi gula sederhana. Pada penelitian ini dilakukan ekstraksi, karakterisasi, uji sitotoksisitas rafinat fukoidan, dan uji aktifitas inhibisi rafinat fukoidan dan hidrolisatnya dari Sargassum polycystum sebagai kandidat inhibitor alami untuk terapi T2DM. Metode penelitian mencakup ekstraksi, karakterisasi, hidrolisis rafinat fukoidan dengan trikloroasetat (TCA) 4%, uji aktivitas inhibisi terhadap enzim α-amilase, dan uji sitotoksisitas pada sel vero. Rafinat fukoidan dihidrolisis dalam dua kondisi inkubasi yang berbeda, yaitu 4 jam pada suhu 80-90 ℃ dan 24 jam pada suhu ruang. Hasil karakterisasi UV-Vis menunjukan adanya puncak serapan khas untuk fukoidan pada λ 267 nm. Analisis FTIR menunjukkan serapan khas golongan senyawa karbohidrat pada panjang gelombang 1041 cm-1 dan puncak serapan khas gugus sulfat pada 1245 cm-1 yang mengkonfirmasi adanya polisakarida sulfat di dalam rafinat fukoidan. Hasil uji menunjukan total karbohidrat, total sulfat, dan aktivitas inhibisi rafinat fukoidan terhadap enzim α-amilase pada penderita T2DM masing-masing sebesar 30,38%; 3,05%; dan 71,27%. Hidrolisat rafinat fukoidan 4 jam pada suhu 80-90 ℃ dan 24 jam pada suhu ruang memiliki total karbohidrat sebesar 34,76% dan 18,62% dengan, total sulfat 5,46% dan 2,38%. Hasil uji sitotoksisitas rafinat fukoidan pada sel vero menunjukan nilai persentase sitotoksisitas terbaik pada konsentrasi maksimum 100 μg/ml sebesar 37,76%. Aktivitas inhibisi hidrolisat rafinat fukoidan terhadap enzim α-amilase pada penderita T2DM sebesar 86,94% dan 62,19%. Berdasarkan hasil penelitian dapat disimpulkan bahwa rafinat fukoidan berpotensi sebagai inhibitor alami untuk terapi T2DM. Type-2 diabetes mellitus (T2DM) is a chronic metabolic disease characterized by high blood sugar levels. Inhibition of α-amylase enzyme can slow down the hydrolysis rate of carbohydrates into simple sugars. This study employed extraction, characterization, cytotoxicity test of fucoidan raffinate, and inhibitory activity of fucoidan raffinate and its hydrolysate derived from Sargassum polycystum as a natural inhibitor candidate for T2DM therapy were conducted. The research methods included extraction, characterization, hydrolysis of fucoidan raffinate with 4% trichloroacetate (TCA), inhibition activity test against α-amylase enzyme, and cytotoxicity test on vero cells. Fucoidan raffinate was hydrolyzed in two different incubation conditions, namely 4 hours at 80-90 ℃ and 24 hours at room temperature. UV-Vis characterization results showed a typical absorption peak for fucoidan at λ 267 nm. FTIR analysis showed typical absorption of carbohydrate compound group at wavelength of 1041 cm-1 and typical absorption peak of sulfate group at 1245 cm-1 which confirmed the presence of sulfate polysaccharide in fucoidan raffinate. The test results showed that the total carbohydrate, total sulfate, and inhibitory activity of fucoidan raffinate against α-amylase enzyme in patients with T2DM were 30.38%; 3.05%; and 71.27%, respectively. Fucoidan raffinate hydrolysate 4 hours at 80-90 ℃ and 24 hours at room temperature had total carbohydrates of 34.76% and 18.62% with, total sulfate 5.46% and 2.38%. The results of the cytotoxicity test of fucoidan raffinate on vero cells showed the best percentage value of cytotoxicity at a maximum concentration of 100 μg/ml of 37.76%. The inhibition activity of fucoidan raffinate hydrolysate against α-amylase enzyme in T2DM patients was 86.94% and 62.19%. Based on the results of the study, it can be concluded that fucoidan raffinate has the potential as a natural inhibitor for T2DM therapy.

Item Type:	Thesis (S1)
Additional Information:	https://scholar.google.com/citations?user=91QK_8oAAAAJ&hl=id&authuser=1&oi=ao ID SINTA Dosen Pembimbing: Vidia Afina Nuarini, S.Si., M.Si.: 6792209 Heli Siti Halimatul Munawaroh, Ph.D.: 5978104
Uncontrolled Keywords:	diabetes melitus, inhibitor enzim α-amilase, polisakarida sulfat, rafinat fukoidan, Sargassum polycystum α-amylase enzyme inhibitor, diabetes mellitus, fucoidan raffinate, polysaccharide sulfate, Sargassum polycystum
Subjects:	L Education > L Education (General)
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan)
Depositing User:	Alya Nurhidayati
Date Deposited:	17 Sep 2024 07:28
Last Modified:	17 Sep 2024 07:32
URI:	http://repository.upi.edu/id/eprint/124997

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