Ziyan Saputra, - (2024) ISOLASI DAN KARAKTERISASI SENYAWA GOLONGAN LIGNAN DARI FRAKSI N-HEKSANA DAUN PALA (Myristica fragrans Houtt.) DAN AKTIVITAS ANTIOKSIDANNYA. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
Lignan merupakan dimer unit fenilpropanoid (C6C3) yang terbentuk melalui ikatan karbon-karbon pada posisi β-β’ (8-8’), sedangkan varian lain yang terbentuk melalui ikatan selain β-β’ (8-8’) disebut neolignan. Penelitian mengenai lignan menarik perhatian karena strukturnya yang unik dan aktivitas biologisnya yang signifikan, seperti aktivitas antitumor dan antikanker. Salah satu tanaman yang diketahui mengandung lignan adalah Myristica fragrans Houtt. (pala), dimana sebanyak 35 lignan dan 91 neolignan telah diidentifikasi dari berbagai bagian tanaman, seperti fuli, biji, buah, kulit buah, dan kulit batang. Namun, studi terkait isolasi senyawa lignan pada daun M. fragrans masih sangat terbatas. Berdasarkan hal tersebut, penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi senyawa golongan lignan dari fraksi n-heksana daun M. fragrans serta menguji aktivitas antioksidannya. Metode yang digunakan untuk uji aktivitas antioksidan adalah metode DPPH. Proses isolasi metabolit sekunder dilakukan melalui beberapa tahapan, yaitu ekstraksi, fraksinasi, dan pemurnian, dengan menggunakan berbagai teknik kromatografi seperti kromatografi cair vakum (KCV), kromatografi kolom gravitasi (KKG), dan kromatografi lapis tipis preparatif (KLTP). Identifikasi struktur senyawa isolat murni dilakukan melalui analisis spektroskopi UV-Vis, FTIR, dan NMR. Dari proses isolasi ini, berhasil diperoleh dua senyawa neolignan, yaitu eritro-Δ8′-7-hidroksi-3,4,5,3′,5′-pentametoksi-8-O-4′-neolignan atau Raphidecursinol B (15,7 mg) sebagai isolat 1 dan eritro-Δ8′-4,7-dihidroksi-3,3′,5′-trimetoksi-8-O-4′-neolignan (7,1 mg) sebagai isolat 2. Uji aktivitas antioksidan yang dilakukan menunjukkan bahwa nilai IC50 untuk isolat 1 adalah 208,83 ± 2,95 µg/mL, sedangkan untuk isolat 2 adalah 43,99 ± 0,15 µg/mL. Berdasarkan hasil ini, isolat 1 dikategorikan memiliki aktivitas antioksidan yang sangat lemah, sedangkan isolat 2 dikategorikan memiliki aktivitas antioksidan yang sangat kuat. Lignan is a dimer of phenylpropanoid units (C6C3) formed through a carbon-carbon bond at the β-β' (8-8') position, while other variants formed through bonds other than β-β' (8-8') are called neolignans. Research on lignan has garnered attention due to its unique structure and significant biological activities, such as antitumor and anticancer properties. One plant known to contain lignan is Myristica fragrans Houtt. (nutmeg), with 35 lignans and 91 neolignans identified from various parts of the plant, including the aril, seed, fruit, fruit pericarp, and steam bark. However, studies related to the isolation of lignan compounds from M. fragrans leaves are still very limited. Based on this, the present research aims to isolate and characterize lignan compounds from the n-hexane fraction of M. fragrans leaves and to test their antioxidant activity. The method used for antioxidant activity testing is the DPPH method. The process of isolating secondary metabolites was carried out through several stages, including extraction, fractionation, and purification, using various chromatographic techniques such as vacuum liquid chromatography (VLC), gravity column chromatography (GCC), and preparative thin-layer chromatography (PTLC). The structure of the pure isolated compounds was identified through UV-Vis, FTIR, and NMR spectroscopy analysis. From this isolation process, two neolignan compounds were successfully obtained, namely erythro-Δ8′-7-hydroxy-3,4,5,3′,5′-pentamethoxy-8-O-4′-neolignan or Raphidecursinol B (15.7 mg) as isolate 1, and erythro-Δ8′-4,7-dihydroxy-3,3′,5′-trimethoxy-8-O-4′-neolignan (7.1 mg) as isolate 2. Antioxidant activity tests showed that the IC50 value for isolate 1 was 208.83 ± 2.95 µg/mL, while for isolate 2 it was 43.99 ± 0.15 µg/mL. Based on these results, isolate 1 was categorized as having very weak antioxidant activity, while isolate 2 was categorized as having very strong antioxidant activity.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?view_op=list_works&hl=id&user=HEf5iPMAAAAJ SINTA ID: 5979687 SINTA ID: 6729178 |
| Uncontrolled Keywords: | Lignan, neolignan, Myristica fragrans Houtt., daun, antioksidan Lignan, neolignan, Myristica fragrans Houtt., leaves, antioxidant |
| Subjects: | L Education > L Education (General) |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Kimia - S1 > Program Studi Kimia (non kependidikan) |
| Depositing User: | Ziyan Saputra |
| Date Deposited: | 16 Sep 2024 14:45 |
| Last Modified: | 16 Sep 2024 14:45 |
| URI: | http://repository.upi.edu/id/eprint/124943 |
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