Razib Ikbal Alfaris, - (2024) ISOLASI DAN IDENTIFIKASI BAKTERI ENDOFUNGAL JAMUR TIRAM (Pleurotus ostreatus) DAN POTENSINYA SEBAGAI ANTIMIKROBA. S1 thesis, Universitas Pendidikan Indonesia.
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Abstract
Masalah penyakit akibat bakteri Escherichia coli dan fungi Candida albicans terjadi cukup luas dengan fenomena resistensi patogen tersebut terhadap pengobatan yang sudah umum menyebabkan perlu ditemukan alternatif lain. Bakteri endofungal telah banyak dimanfaatkan sebagai penghasil senyawa antibakteri. Kemudahan produksi serta harga ekonomis dan inang yang mudah didapat mendorong dilakukannya pengujian aktivitas antimikroba dari ekstrak bakteri endofungal jamur tiram (Pleurotus ostreatus) terhadap E. coli dan C. albicans. Penelitian didahului dengan isolasi bakteri endofungal jamur tiram, identifikasi sampai tingkat genus, seleksi isolat potensial penghasil senyawa antimikroba, pengukuran kurva tumbuh, ekstraksi, lalu pengujian aktivitas antimikroba. Pengujian aktivitas antimikroba dilakukan dengan uji DDA, MIC, dan MBC dengan perlakuan konsentrasi ekstrak yaitu 40,0 mg/ml, 10,0 mg/ml, dan 2,5 mg/ml dengan kontrol positif berupa ampicillin 50 µg/ml untuk E. coli dan ketoconazole 30 mg/ml untuk C. albicans, serta kontrol negatif berupa DMSO 10%. Hasil identifikasi menunjukkan sebanyak enam isolat memiliki karakteristik mendekati genus Bacillus, empat isolat mendekati Acinetobacter, dua isolat mendekati Neisseria, dan masing-masing satu isolat mendekati Pseudomonas dan Lactobacillus. Diameter zona hambat ekstrak supernatan isolat bakteri endofungal yang berbeda signifikan terhadap E. coli dihasilkan sebanyak dua isolat bakteri pada konsentrasi 40,0 mg/ml. Sementara itu, terdapat empat isolat bakteri yang memiliki daya hambat signifikan terhadap C. albicans pada konsentrasi 40,0 mg/ml. Hasil uji MIC dan MBC dari semua ekstrak supernatan bakteri menunjukkan nilai konsentrasi MIC dan MBC yang sejalan dengan hasil uji DDA. Dari hasil penelitian terbukti bahwa ekstrak metabolit isolat bakteri endofungal P. ostreatus memiliki aktivitas antimikroba terhadap E. coli dan C. albicans. The disease problems caused by Escherichia coli and Candida albicans are quite widespread, with the phenomenon of resistance of these pathogens to common treatments, making it necessary to find other alternatives. Endofungal bacteria have been widely used as producers of antibacterial compounds. The ease of production, economical price, and availability of hosts encouraged testing the antimicrobial activity of the endofungal bacterial extract of oyster mushrooms (Pleurotus ostreatus) against E. coli and C. albicans. The research was proceded by the isolation of oyster mushroom endofungal bacteria, identification to genus level, selection of potential isolates producing antimicrobial compounds, measurement of growth curves, extraction, and testing of antimicrobial activity. Antimicrobial activity testing was carried out using the DDA, MIC, and MBC tests with treatment extract concentrations of 40.0 mg/ml, 10.0 mg/ml, and 2.5 mg/ml with a positive control in the form of ampicillin 50 µg/ml for E. coli and ketoconazole 30 mg/ml for C. albicans, as well as a negative control in the form of 10% DMSO. The identification results showed that six isolates had characteristics close to the Bacillus genus, four isolates were close to Acinetobacter, two isolates were close to Neisseria, and one isolate each was close to Pseudomonas and Lactobacillus. The diameter of the inhibition zone of the supernatant extract of endofungal bacterial isolates which was significantly different from E. coli resulted in two bacterial isolates at concentration of 40.0 mg/ml. Meanwhile, four bacterial isolates had significant inhibitory power against C. albicans at a concentration of 40.0 mg/ml. The MIC and MBC test results of all bacterial supernatant extracts showed concentration values were in line with the DDA test results. From the research results, it was proven that the metabolite extract of the endofungal bacterial isolate P. ostreatus had antimicrobial activity against E. coli and C. albicans.
| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | https://scholar.google.com/citations?view_op=list_works&hl=en&user=RsgAKX4AAAAJ ID SINTA Dosen pembimbing Any Fitriani: 6004273 Peristiwati: 6146081 |
| Uncontrolled Keywords: | Aktivitas antimikroba terhadap bakteri Escherichia coli dan fungi Candida albicans dari ekstrak metabolit bakteri endofungal jamur edible Antimicrobial activity against Escherichia coli bacteria and Candida albicans fungi from metabolite extract of endofungal bacteria from edible mushroom |
| Subjects: | L Education > L Education (General) Q Science > QR Microbiology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Biologi > Program Studi Biologi (non kependidikan) |
| Depositing User: | Razib Ikbal Alfaris |
| Date Deposited: | 10 Sep 2024 18:44 |
| Last Modified: | 10 Sep 2024 18:44 |
| URI: | http://repository.upi.edu/id/eprint/123666 |
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