Fiqha Azkiya, - (2024) ISOLASI BAKTERI ENDOFIT DAUN PUCUK MERAH (Syzygium myrtifolium Walp.) DAN POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Cutibacterium acnes. S1 thesis, Universitas Pendidikan Indonesia.
Abstract
ABSTRAK Tanaman pucuk merah (Syzygium myrtifolium Walp.) merupakan tanaman perdu yang berasal dari Asia Tenggara. Daun tanaman pucuk merah diketahui mengandung bakteri endofit yang mampu menghambat pertumbuhan bakteri. Cutibacterium acnes merupakan salah satu bakteri penyebab jerawat. Tujuan dari penelitian ini adalah untuk mengetahui genus bakteri endofit pada daun S. myrtifolium Walp. dan potensinya sebagai antibakteri terhadap C. acnes. Penelitian diawali dari proses pengambilan sampel, isolasi bakteri endofit, pembiakan kultur murni bakteri endofit, identifikasi bakteri endofit, pembuatan kurva tumbuh bakteri, ekstraksi supernatan bakteri endofit, uji aktivitas antibakteri mencakup Disc Diffusion Assay (DDA), Minimal Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) serta tahap terakhir yaitu analisis data. Bakteri endofit daun S. myrtifolium Walp. teridentifikasi memiliki kemiripan dengan genus Bacillus, Pseudomonas, Micrococcus, Acinetobacter, Neisseria, Arthrobacter, dan Enterobacter. Aktivitas antibakteri pada ekstrak supernatan bakteri endofit isolat M1 dan M2 terhadap bakteri patogen C. acnes menghasilkan diameter zona hambat yang berbeda signifikan. Ekstrak supernatan bakteri endofit isolat M2 dengan konsentrasi 50 mg/mLmenghasilkan diameter zona hambat terbesar, yaitu 14,67 mm pada isolat M1 dan 15,33 mm pada isolat M2. Aktivitas antibakteri pada ekstrak supernatan bakteri endofit isolat M1 dan M2 terhadap bakteri patogen C. acnes, memiliki nilai MIC yaitu 2,5 mg/mLdan nilai MBC yaitu 3,0 mg/mL Hasil tersebut menunjukkan bahwa ekstrak supernatan bakteri endofit daun S. myrtifolium Walp. memiliki aktivitas antibakteri terhadap C. acnes. Penelitian ini diharapkan dapat menjadi acuan dalam pengembangan obat antibakteri terhadap C. acnes. ABSTRACT The redbud trees (Syzygium myrtifolium Walp) is a shrub that originates from Southeast Asia. The leaves of the red shoot plant are known to contain endophytic bacteria which can inhibit bacterial growth. Cutibacterium acnes is one of the bacteria that causes acne. The aim of this research was to determine the genus of endophytic bacteria on the leaves of S. myrtifolium Walp. and its potential as an antibacterial against C. acnes. The study began with the process of sampling, isolation of endophytic bacteria, cultivation of pure cultures of endophytic bacteria, identification of endophytic bacteria, creation of growth curves of bacteria, extraction of endophytic bacterial supernatants, antibacterial activity tests including Disc Diffusion Assay (DDA), Minimal Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and the final stage is data analysis. Endophytic bacteria in S. myrtifolium Walp. leaves were identified as having similarities with the genera Bacillus, Pseudomonas, Micrococcus, Acinetobacter, Neisseria, Arthrobacter, and Enterobacter. Antibacterial activity of the supernatant extract of endophytic bacteria isolates M1 and M2 against pathogenic bacteria C. acnes produced significantly different inhibition zone diameters. The supernatant extract of endophytic bacteria isolate M2 with a concentration of 50 mg/mL produced the largest inhibition zone diameter, which was 14.67 mm in isolate M1 and 15.33 mm in isolate M2. The antibacterial activity of the supernatant extract of endophytic bacteria isolates M1 and M2 against pathogenic bacteria C. acnes, had a MIC value of 2.5 mg/mL and an MBC value of 3.0 mg/mL. These results indicate that the supernatant extract of endophytic bacteria from S. myrtifolium Walp. leaves has antibacterial activity against C. acnes. This study is expected to be a reference in the development of antibacterial drugs against C. acnes.
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| Item Type: | Thesis (S1) |
|---|---|
| Additional Information: | ID SINTA Dosen pembimbing Any Fitriani: 6004273 Peristiwati: 6146081 |
| Uncontrolled Keywords: | Antibakteri, bakteri endofit, Cutibacterium acnes, Syzygium myrtifolium Antibacterial, endophytic bacteria, Cutibacterium acnes, Syzygium myrtifolium |
| Subjects: | L Education > L Education (General) Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
| Divisions: | Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Program Studi Biologi - S1 > Program Studi Biologi (non kependidikan) |
| Depositing User: | Fiqha Azkiya |
| Date Deposited: | 13 Sep 2024 06:38 |
| Last Modified: | 13 Sep 2024 06:38 |
| URI: | http://repository.upi.edu/id/eprint/123339 |
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