EVALUASI AKTIVITAS ANTIBAKTERI EKSTRAK FENOLIK Spirulina platensis SEBAGAI KANDIDAT SEDIAAN SALEP LUKA DIABETES

Deaniar Hafilah, - (2023) EVALUASI AKTIVITAS ANTIBAKTERI EKSTRAK FENOLIK Spirulina platensis SEBAGAI KANDIDAT SEDIAAN SALEP LUKA DIABETES. S1 thesis, Universitas Pendidikan Indonesia.

	Text S_KIM_1909295_Title.pdf Download (522kB)
	Text S_KIM_1909295_Chapter 1.pdf Download (177kB)
	Text S_KIM_1909295_Chapter 2.pdf Restricted to Staf Perpustakaan Download (606kB)
	Text S_KIM_1909295_Chapter 3.pdf Download (625kB)
	Text S_KIM_1909295_Chapter 4.pdf Restricted to Staf Perpustakaan Download (1MB)
	Text S_KIM_1909295_Chapter 5.pdf Download (94kB)
	Text S_KIM_1909295_Appendix.pdf Restricted to Staf Perpustakaan Download (360kB)

Abstract

Infeksi bakteri pada luka diabetes dapat memperparah kondisi luka yang jika dibiarkan dapat mengakibatkan amputasi bahkan kematian. Pada penelitian ini, dilakukan analisis potensi antibakteri dari ekstrak fenolik Spirulina platensis sebagai kandidat bahan salep luka diabetes menggunakan pendekatan in vitro dan in silico. Pengujian in vitro diawali dengan penentuan karakteristik ekstrak fenolik Spirulina platensis yang ditentukan menggunakan skrining fitokimia, penentuan gugus fungsi menggunakan FTIR, dan penentuan total kadar fenolik. Potensi antibakteri ekstrak fenolik Spirulina platensis ditentukan melalui penentuan MIC (Minimum Inhibitory Concentration) dan MBC (Minimum Bactericidal Concentration). Pengujian fitokimia dan FTIR menunjukkan bahwa ekstrak Spirulina platensis mengandung senyawa fenolik dengan total kandungan sebesar 198,43 ± 3,95 mg GAE/g ekstrak. Nilai MIC dan MBC terhadap Staphylococcus aureus masing-masing yaitu 500 ppm dan 1000 ppm, MIC dan MBC terhadap Staphylococcus epidermidis yaitu 500 ppm, MIC dan MBC terhadap Pseudomonas aeruginosa masing-masing sebesar 62,5 ppm dan 125 ppm. Pada pengujian in silico, dilakukan visualisasi interaksi antara senyawa fenolik terhadapi Penicillin binding Protein (PBP) enzim yang berperan dalam sintesis dan pemeliharaan struktur dinding sel. Hasil simulasi menunjukkan bahwa ligan dari kelompok senyawa fenolik berinteraksi dengan enzim PBP melalui ikatan hidrogen, hidrofobik, dan van der Waals dengan energi afinitas pengikatan beberapa senyawa fenolik yaitu asam ferulat, asam galat, asam kafeat, asam klorogenat, asam siringat, asam vanilat, dan asam p-kumarat masing-masing sebesar -5,8 kkal/mol, -5,9 kkal/mol, -6,0 kkal/mol, -7,2 kkal/mol, -5,9 kkal/mol, -5,8 kkal/mol, dan -5,5 kkal/mol. Beberapa senyawa fenolik dalam Spirulina platensis diprediksi memiliki aktivitas inhibisi yang lebih besar dari antibiotik Amoxicillin yang memiliki energi afinitas pengikatan dengan enzim PBP sebesar -4,9 kkal/mol. Senyawa fenolik dalam Spirulina platensis diprediksi dapat menghambat enzim PBP melalui mekanisme inhibisi kompetitif. Berdasarkan hasil penelitian dapat disimpulkan bahwa senyawa fenolik dari ekstrak fenolik Spirulina platensis memiliki potensi sebagai kandidat bahan antibakteri yang dapat mencegah infeksi pada luka diabetes. The wound healing of diabetic ulcers can be slowed down due to bacterial infection., If this situation left untreated, it can lead to amputation or even death. This study aims to explore the antibacterial activity of phenolic extracts derived from Spirulina platensis an active material for diabetic wound dressing using in vitro and in silico approaches. The in vitro testing began with determining the characteristics of the phenolic extract from Spirulina platensis through phytochemical screening, functional group determination using FTIR, and total phenolic content assessment. The antibacterial potential of the phenolic extract from Spirulina platensis was evaluated by determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Phytochemical and FTIR analyses revealed that the Spirulina platensis extract contained phenolic compounds with a total content of 198.42 ± 3.95 mg GAE/g of extract. The MIC and MBC values against Staphylococcus aureus were 500 ppm and 1000 ppm, against Staphylococcus epidermidis were both 500 ppm, and against Pseudomonas aeruginosa were 62.5 ppm and 125 ppm, respectively. The in silico simulation identified, the interaction among phenolic compounds and the Penicillin binding Protein (PBP). PBP is an enzyme involved in cell wall synthesis and maintenance. Simulation results showed that ligands from the phenolic compound group interacted with the PBP enzyme through hidrogen bonding, hydrophobic, and van der Waals interactions, with binding affinity energies for some phenolic compounds such as ferulic acid, gallic acid, caffeic acid, chlorogenic acid, syringic acid, vanillic acid, and p-coumaric acid being -5.8 kcal/mol, -5.9 kcal/mol, -6.0 kcal/mol, -7.2 kcal/mol, -5.9 kcal/mol, -5.8 kcal/mol, and -5.5 kcal/mol, respectively. Several phenolic compounds in Spirulina platensis were predicted to have greater inhibitory activity than the antibiotik Amoxicillin, which exhibited a binding affinity energy with the PBP enzyme of -4.9 kcal/mol. Phenolic compounds in Spirulina platensis are predicted to inhibit the PBP enzyme through a mechanism of competitive inhibition. Based on the research findings, it can be concluded that phenolic compounds from the phenolic extract of Spirulina platensis have the potential to be developed as candidate antibacterial agents that can prevent infection in diabetic ulcer.

Item Type:	Thesis (S1)
Additional Information:	ID Sinta Dosen Pembimbing: 5978104 HELI SITI HALIMATUL MUNAWAROH 5983442 GUN GUN GUMILAR
Uncontrolled Keywords:	antibakteri, fenolik, luka diabetes, Penicillin binding Protein, Spirulina platensis antibacterial, diabetic ulcers, phenolic, Penicillin binding Protein, Spirulina platensis
Subjects:	Q Science > QD Chemistry
Divisions:	Fakultas Pendidikan Matematika dan Ilmu Pengetahuan Alam > Jurusan Pendidikan Kimia > Program Studi Kimia (non kependidikan)
Depositing User:	Deaniar Hafilah
Date Deposited:	26 Sep 2023 07:57
Last Modified:	26 Sep 2023 07:57
URI:	http://repository.upi.edu/id/eprint/108308

Actions (login required)

View Item